- PDB-1mk3: SOLUTION STRUCTURE OF HUMAN BCL-W PROTEIN -
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基本情報
登録情報
データベース: PDB / ID: 1mk3
タイトル
SOLUTION STRUCTURE OF HUMAN BCL-W PROTEIN
要素
Apoptosis regulator Bcl-W
キーワード
APOPTOSIS / Bcl-w protein / apoptotis
機能・相同性
機能・相同性情報
negative regulation of mitochondrial membrane permeability / Sertoli cell proliferation / BH domain binding / Bcl-2 family protein complex / negative regulation of release of cytochrome c from mitochondria / cellular response to glycine / negative regulation of intrinsic apoptotic signaling pathway / extrinsic apoptotic signaling pathway in absence of ligand / cellular response to estradiol stimulus / response to ischemia ...negative regulation of mitochondrial membrane permeability / Sertoli cell proliferation / BH domain binding / Bcl-2 family protein complex / negative regulation of release of cytochrome c from mitochondria / cellular response to glycine / negative regulation of intrinsic apoptotic signaling pathway / extrinsic apoptotic signaling pathway in absence of ligand / cellular response to estradiol stimulus / response to ischemia / cellular response to amyloid-beta / intrinsic apoptotic signaling pathway in response to DNA damage / disordered domain specific binding / spermatogenesis / regulation of apoptotic process / mitochondrial outer membrane / protein heterodimerization activity / protein-containing complex binding / negative regulation of apoptotic process / protein homodimerization activity / identical protein binding / cytosol 類似検索 - 分子機能
Apoptosis regulator, Bcl-W / Apoptosis regulator, Bcl-2, BH4 motif, conserved site / Apoptosis regulator, Bcl-2 family BH4 motif signature. / Apoptosis regulator, Bcl-2 protein, BH4 / Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2 family BH4 motif profile. / BH4 Bcl-2 homology region 4 / Blc2-like / Apoptosis Regulator Bcl-x / Apoptosis regulator, Bcl-2, BH1 motif, conserved site ...Apoptosis regulator, Bcl-W / Apoptosis regulator, Bcl-2, BH4 motif, conserved site / Apoptosis regulator, Bcl-2 family BH4 motif signature. / Apoptosis regulator, Bcl-2 protein, BH4 / Bcl-2 homology region 4 / Apoptosis regulator, Bcl-2 family BH4 motif profile. / BH4 Bcl-2 homology region 4 / Blc2-like / Apoptosis Regulator Bcl-x / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl-2 family / Bcl-2, Bcl-2 homology region 1-3 / Bcl2-like / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
Text: The structure was determined using triple-resonance NMR spectroscopy
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1.4 mM Bcl-w protein U-15N,13C, 20 mM phosphate buffer
90% H2O/10% D2O
2
1.3 mM Bcl-w protein U-15N, 20 mM phosphate buffer
90% H2O/10% D2O
3
0.5 mM Bcl-w protein U-15N, 6 MG/ML PF1-PHAGE
90% H2O/10% D2O
試料状態
イオン強度: 10 mM NaCl / pH: 7.3 / 圧: 1 atm / 温度: 298 K
結晶化
*PLUS
手法: other / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
500
1
Varian INOVA
Varian
INOVA
800
2
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解析
NMR software
名称
バージョン
開発者
分類
CNS
Brunger
collection
CNS
Brunger
解析
CNS
Brunger
データ解析
CNS
1.1
Brungeretal.
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: Structure refined by using standard protocol in CNS with restraints from NOE distances, backbone torsion angles, hydrogen bonds and residual dipolar couplings
代表構造
選択基準: closest to the average,lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the least restraint violations,structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 10