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- PDB-1mit: RECOMBINANT CUCURBITA MAXIMA TRYPSIN INHIBITOR V (RCMTI-V) (NMR, ... -

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Basic information

Entry
Database: PDB / ID: 1mit
TitleRECOMBINANT CUCURBITA MAXIMA TRYPSIN INHIBITOR V (RCMTI-V) (NMR, MINIMIZED AVERAGE STRUCTURE)
ComponentsTRYPSIN INHIBITOR V
KeywordsSERINE PROTEASE INHIBITOR (RCMTI-V)
Function / homology
Function and homology information


serine-type endopeptidase inhibitor activity / response to wounding
Similarity search - Function
Trypsin Inhibitor V, subunit A / Proteinase inhibitor I13, potato inhibitor I / Proteinase inhibitor I13, potato inhibitor I superfamily / Potato inhibitor I family / Potato inhibitor I family signature. / Trypsin Inhibitor V; Chain A / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Inhibitor of trypsin and hageman factor
Similarity search - Component
Biological speciesCucurbita maxima (winter squash)
MethodSOLUTION NMR
AuthorsCai, M. / Gong, Y. / Huang, Y. / Liu, J. / Prakash, O. / Wen, L. / Wen, J.J. / Huang, J.-K. / Krishnamoorthi, R.
Citation
Journal: Biochemistry / Year: 1996
Title: Solution structure and backbone dynamics of recombinant Cucurbita maxima trypsin inhibitor-V determined by NMR spectroscopy.
Authors: Liu, J. / Prakash, O. / Cai, M. / Gong, Y. / Huang, Y. / Wen, L. / Wen, J.J. / Huang, J.K. / Krishnamoorthi, R.
#1: Journal: Biochemistry / Year: 1995
Title: Investigation of Solution Conformation of Cucurbita Maxima Trypsin Inhibitor V from Pumpkin Seeds Via NMR Spectroscopy
Authors: Cai, M. / Gong, Y. / Kao, J.L.F. / Krishnamoorthi, R.
#2: Journal: Protein Expr.Purif. / Year: 1993
Title: Chemical Synthesis, Molecular Cloning, Over Expression, and Site-Directed Mutagenesis of the Gene Coding for Pumpkin (Cucurbita Maxima) Trypsin Inhibitor Cmti-V
Authors: Wen, L. / Kim, S. / Tinn, T.T. / Huang, J.-K. / Krishnamoorthi, R. / Gong, Y. / Lwin, Y.N. / Kyin, S.
#3: Journal: FEBS Lett. / Year: 1990
Title: A New Protein Inhibitor of Trypsin and Activated Hageman Factor from Pumpkin (Cucurbita Maxima) Seeds
Authors: Krishnamoorthi, R. / Gong, Y. / Richardson, M.
History
DepositionOct 26, 1995Processing site: BNL
Revision 1.0Apr 3, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4Oct 30, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TRYPSIN INHIBITOR V


Theoretical massNumber of molelcules
Total (without water)7,4321
Polymers7,4321
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein TRYPSIN INHIBITOR V / RCMTI-V


Mass: 7431.653 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Cucurbita maxima (winter squash) / Organ: SEED / Production host: Escherichia coli (E. coli) / References: UniProt: P19873
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other

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Processing

NMR ensembleConformers submitted total number: 1

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