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Open data
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Basic information
Entry | Database: PDB / ID: 1mhy | |||||||||
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Title | METHANE MONOOXYGENASE HYDROXYLASE | |||||||||
![]() | (METHANE MONOOXYGENASE HYDROXYLASE) x 3 | |||||||||
![]() | OXIDOREDUCTASE / MONOOXYGENASE / NADP / ONE-CARBON METABOLISM | |||||||||
Function / homology | ![]() methane metabolic process / methane monooxygenase (soluble) / : / : / : / one-carbon metabolic process / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Elango, N. / Radhakrishnan, R. / Froland, W.A. / Waller, B.J. / Earhart, C.A. / Lipscomb, J.D. / Ohlendorf, D.H. | |||||||||
![]() | ![]() Title: Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b Authors: Elango, N. / Radhakrishnan, R. / Froland, W.A. / Wallar, B.J. / Earhart, C.A. / Lipscomb, J.D. / Ohlendorf, D.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 310.1 KB | Display | ![]() |
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PDB format | ![]() | 247.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 382.4 KB | Display | ![]() |
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Full document | ![]() | 392.5 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 37.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mhzC ![]() 1mmoS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 45165.145 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P27354, methane monooxygenase (soluble) | ||
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#2: Protein | Mass: 59513.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P27353, methane monooxygenase (soluble) | ||
#3: Protein | Mass: 19358.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P27355, methane monooxygenase (soluble) | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 54 % | |||||||||||||||||||||||||
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Crystal grow | Details: FROLAND W.A. ET AL. (1994) J. MOL. BIOL. VOL. 236:379-381. | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: used to seeding, Froland, W.A., (1994) J. Mol. Biol., 236, 379. PH range low: 7.8 / PH range high: 7.4 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 291 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 10, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Num. obs: 87489 / % possible obs: 96.8 % / Observed criterion σ(I): 1 / Redundancy: 4 % / Rmerge(I) obs: 0.075 / Net I/σ(I): 12.5 |
Reflection | *PLUS Num. measured all: 391667 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: CHAINS B, D, AND G OF PDB ENTRY 1MMO Resolution: 2→5 Å / σ(F): 2
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Displacement parameters | Biso mean: 23.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.15 Å / Luzzati d res low obs: 5 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→5 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.145 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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