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- PDB-1fz0: METHANE MONOOXYGENASE HYDROXYLASE, FORM II MIXED-VALENT GROWN ANA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1fz0 | ||||||
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Title | METHANE MONOOXYGENASE HYDROXYLASE, FORM II MIXED-VALENT GROWN ANAEROBICALLY | ||||||
![]() | (METHANE MONOOXYGENASE COMPONENT A, ...) x 3 | ||||||
![]() | OXIDOREDUCTASE / dinuclear iron center / monooxygenase | ||||||
Function / homology | ![]() methane metabolic process / methane monooxygenase (soluble) / : / : / : / one-carbon metabolic process / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Whittington, D.A. / Lippard, S.J. | ||||||
![]() | ![]() Title: Crystal structures of the soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath) demonstrating geometrical variability at the dinuclear iron active site. Authors: Whittington, D.A. / Lippard, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 463.3 KB | Display | ![]() |
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PDB format | ![]() | 375.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 482 KB | Display | ![]() |
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Full document | ![]() | 526.5 KB | Display | |
Data in XML | ![]() | 90.2 KB | Display | |
Data in CIF | ![]() | 129.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1fyzC ![]() 1fz1C ![]() 1fz2C ![]() 1fz3C ![]() 1fz4C ![]() 1fz5C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-METHANE MONOOXYGENASE COMPONENT A, ... , 3 types, 6 molecules ABCDEF
#1: Protein | Mass: 60719.113 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P22869, methane monooxygenase (soluble) #2: Protein | Mass: 45184.660 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P18798, methane monooxygenase (soluble) #3: Protein | Mass: 19879.732 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P11987, methane monooxygenase (soluble) |
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-Non-polymers , 3 types, 1350 molecules ![](data/chem/img/FE2.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-FE2 / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.42 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 8000, CaCl2, MOPS, 1,10-decanedioic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 98 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.07→50 Å / Num. all: 166257 / Num. obs: 165782 / % possible obs: 99.5 % / Observed criterion σ(I): 2 / Redundancy: 10.5 % / Biso Wilson estimate: 12.6 Å2 / Rmerge(I) obs: 0.082 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.07→2.11 Å / Redundancy: 4 % / Rmerge(I) obs: 0.451 / % possible all: 98.8 |
Reflection | *PLUS Num. measured all: 1736424 |
Reflection shell | *PLUS % possible obs: 98.8 % / Mean I/σ(I) obs: 3 |
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Processing
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Refinement | Resolution: 2.07→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.07→30 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 30 Å / σ(F): 0 / % reflection Rfree: 3.6 % / Rfactor Rfree: 0.23 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 30.2 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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