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Open data
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Basic information
Entry | Database: PDB / ID: 1mhx | ||||||
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Title | Crystal Structures of the redesigned protein G variant NuG1 | ||||||
![]() | immunoglobulin-binding protein G | ||||||
![]() | IMMUNE SYSTEM / alpha-beta protein / redesigned first beta-hairpin | ||||||
Function / homology | ![]() Protein L, Ig light chain-binding / Protein L b1 domain / IgG-binding B / B domain / Ubiquitin-like (UB roll) - #10 / Ubiquitin-like (UB roll) / Roll / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nauli, S. / Kuhlman, B. / Le Trong, I. / Stenkamp, R.E. / Teller, D.C. / Baker, D. | ||||||
![]() | ![]() Title: Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2 Authors: Nauli, S. / Kuhlman, B. / Le Trong, I. / Stenkamp, R.E. / Teller, D.C. / Baker, D. | ||||||
History |
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Remark 999 | SEQUENCE THE SEQUENCE DIFFERS FROM PIR ENTRY A45063 AT RESIDUES 15-25 (PIR RESIDUES 334-344) ...SEQUENCE THE SEQUENCE DIFFERS FROM PIR ENTRY A45063 AT RESIDUES 15-25 (PIR RESIDUES 334-344) BECAUSE THE AUTHORS REDESIGNED THE FIRST HAIRPIN. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 26.2 KB | Display | ![]() |
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PDB format | ![]() | 16.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421.9 KB | Display | ![]() |
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Full document | ![]() | 422.7 KB | Display | |
Data in XML | ![]() | 5.8 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mi0C ![]() 1pgaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 7359.115 Da / Num. of mol.: 1 / Fragment: Redesigned B1 domain / Mutation: T58A Source method: isolated from a genetically manipulated source Details: Redesigned first beta-hairpin, variant NuG1 / Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.53 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: n-propanol, sodium formate, tris-hcl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 20, 2001 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→22.3 Å / Num. obs: 6273 / % possible obs: 95.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2.2 |
Reflection shell | Resolution: 1.8→1.86 Å / % possible all: 92.5 |
Reflection | *PLUS Num. obs: 6383 / Rmerge(I) obs: 0.116 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1pga Resolution: 1.8→22.3 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.8→22.3 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS Type: x_bond_d / Dev ideal: 0.006 |