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Open data
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Basic information
Entry | Database: PDB / ID: 1mfk | ||||||||||||||||||
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Title | Structure of Prokaryotic SECIS mRNA Hairpin | ||||||||||||||||||
![]() | 5'-R(P*![]() RNA / RNA tetraloop / (A/U)GNN tetraloop family / SelB | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / simulated annealing | ![]() Fourmy, D. / Guittet, E. / Yoshizawa, S. | ![]() ![]() Title: Structure of Prokaryotic SECIS mRNA Hairpin and its Interaction with Elongation Factor SELB Authors: Fourmy, D. / Guittet, E. / Yoshizawa, S. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 284.8 KB | Display | ![]() |
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PDB format | ![]() | 238.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 7386.423 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: THE SEQUENCE is from ESCHERICHIA COLI. T7 RNA polymerase in vitro transcription. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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Sample preparation
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: 260 NOE-derived distance constraints, 72 dihedral angle restraints | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |