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Yorodumi- PDB-1mal: STRUCTURAL BASIS FOR SUGAR TRANSLOCATION THROUGH MALTOPORIN CHANN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mal | ||||||
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Title | STRUCTURAL BASIS FOR SUGAR TRANSLOCATION THROUGH MALTOPORIN CHANNELS AT 3.1 ANGSTROMS RESOLUTION | ||||||
Components | MALTOPORIN | ||||||
Keywords | OUTER MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information maltodextrin transmembrane transporter activity / maltose transporting porin activity / polysaccharide transport / porin activity / maltodextrin transmembrane transport / pore complex / carbohydrate transmembrane transporter activity / monoatomic ion transport / cell outer membrane / virus receptor activity / DNA damage response Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3.1 Å | ||||||
Authors | Schirmer, T. | ||||||
Citation | Journal: Science / Year: 1995 Title: Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution. Authors: Schirmer, T. / Keller, T.A. / Wang, Y.F. / Rosenbusch, J.P. #1: Journal: J.Mol.Biol. / Year: 1990 Title: Crystallization and Preliminary X-Ray Characterization of Maltoporin from E.Coli Authors: Stauffer, K.A. / Page, M.P.G. / Hardmeyer, A. / Keller, T.A. / Pauptit, R.A. | ||||||
History |
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Remark 700 | SHEET THE SHEET PRESENTED AS *A* ON SHEET RECORDS BELOW IS ACTUALLY A EIGHTEEN-STRANDED BETA-BARREL. ...SHEET THE SHEET PRESENTED AS *A* ON SHEET RECORDS BELOW IS ACTUALLY A EIGHTEEN-STRANDED BETA-BARREL. THIS IS REPRESENTED BY A NINETEEN-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THERE IS A BETA-BULGE IN STRAND 6 (RESIDUES 131 - 132) AND IN STRAND 8 (RESIDUES 177 - 178). |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mal.cif.gz | 247.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1mal.ent.gz | 209.4 KB | Display | PDB format |
PDBx/mmJSON format | 1mal.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ma/1mal ftp://data.pdbj.org/pub/pdb/validation_reports/ma/1mal | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 361 | ||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 47425.785 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Organelle: OUTER MEMBRANE / References: UniProt: P02943 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.48 Å3/Da / Density % sol: 77.56 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Detector | Date: Jan 7, 1994 |
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Radiation | Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.1→8 Å / Num. obs: 51039 / % possible obs: 95.5 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Rmerge(I) obs: 0.088 |
Reflection | *PLUS Rmerge(I) obs: 0.088 |
-Processing
Software |
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Refinement | Resolution: 3.1→8 Å / σ(F): 0 Details: ATOMS THAT ARE NOT DEFINED BY ELECTRON-DENSITY HAVE BEEN ASSIGNED AN OCCUPANCY OF 0.0. RESIDUE ASP 78 IS CLOSE TO THE LOCAL THREE-FOLD AXIS; INTERACTIONS WITH ITS LOCAL SYMMETRY MATES ARE ...Details: ATOMS THAT ARE NOT DEFINED BY ELECTRON-DENSITY HAVE BEEN ASSIGNED AN OCCUPANCY OF 0.0. RESIDUE ASP 78 IS CLOSE TO THE LOCAL THREE-FOLD AXIS; INTERACTIONS WITH ITS LOCAL SYMMETRY MATES ARE MEDIATED BY AN UNMODELED ATOM OR MOLECULE (PROBABLY A CATION) THAT IS LOCATED ON THE THREE-FOLD AXIS AS JUDGED BY THE APPARENT STRONG ELECTRON DENSITY.
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Displacement parameters | Biso mean: 34.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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