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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1lpv | ||||||
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タイトル | DROSOPHILA MELANOGASTER DOUBLESEX (DSX), NMR, 18 STRUCTURES | ||||||
![]() | Doublesex protein | ||||||
![]() | TRANSCRIPTION / DROSOPHILA MELANOGASTER / DNA BINDING / GENE REGULATION | ||||||
機能・相同性 | ![]() imaginal disc-derived male genitalia development / male analia development / female analia development / female sex differentiation / sex-specific pigmentation / female somatic sex determination / imaginal disc-derived female genitalia development / sex comb development / negative regulation of developmental pigmentation / somatic sex determination ...imaginal disc-derived male genitalia development / male analia development / female analia development / female sex differentiation / sex-specific pigmentation / female somatic sex determination / imaginal disc-derived female genitalia development / sex comb development / negative regulation of developmental pigmentation / somatic sex determination / genital disc development / male sex differentiation / courtship behavior / male courtship behavior, veined wing generated song production / sex determination / male courtship behavior / sex differentiation / axon midline choice point recognition / RNA polymerase II transcription regulatory region sequence-specific DNA binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / zinc ion binding / nucleus 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
![]() | Zhu, L. / Wilken, J. / Phillips, N. / Narendra, U. / Chan, G. / Stratton, S. / Kent, S. / Weiss, M.A. | ||||||
![]() | ![]() タイトル: Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers. 著者: Zhu, L. / Wilken, J. / Phillips, N.B. / Narendra, U. / Chan, G. / Stratton, S.M. / Kent, S.B. / Weiss, M.A. #1: ![]() タイトル: The Drosophila Doublesex Proteins Share a Novel Zin Finger Related DNA-Binding Domain 著者: Erdman, S.E. / Burtis, K.C. #2: ![]() タイトル: Evidence for Evolutionary Conservation of Sex-Determining Genes 著者: Raymond, C.S. / Shamu, C.E. / Shen, M.M. / Seifert, K.J. / Hirsch, B. / Hodgkin, J. / Zarkower, D. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 311.7 KB | 表示 | ![]() |
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PDB形式 | ![]() | 256.4 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 344.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 480.8 KB | 表示 | |
XML形式データ | ![]() | 33.3 KB | 表示 | |
CIF形式データ | ![]() | 50.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 6125.327 Da / 分子数: 1 / 由来タイプ: 合成 詳細: The sequence naturally occurs in Drosophila melanogaster (Fruit fly). 参照: UniProt: P23023 |
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#2: 化合物 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: NMR EXPERIMENTS WERE CONDUCTED ON VARIAN INOVA 600 MHZ SPECTROMETERS. SOFTWARE VNMR5.1 AND FELIX WERE USED TO PROCESS NMR DATA. STRUCTURE DETERMINATION WAS PERFORMED ON A SILICON GRAPHICS WORK ...Text: NMR EXPERIMENTS WERE CONDUCTED ON VARIAN INOVA 600 MHZ SPECTROMETERS. SOFTWARE VNMR5.1 AND FELIX WERE USED TO PROCESS NMR DATA. STRUCTURE DETERMINATION WAS PERFORMED ON A SILICON GRAPHICS WORK STATION. IN 50 MM DEUTERATED TRIS, 10 MM DEUTERATED DTT. |
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試料調製
試料状態 | pH: 6.5 / 温度: 298 K |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 600 MHz |
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解析
NMR software |
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精密化 | 手法: DISTANCE GEOMETRY, SIMULATED ANNEALING / ソフトェア番号: 1 詳細: MOLECULAR MODELS WERE OBTAINED BY DGII IMPLEMENTED IN INSIGHT II (BIOSYM INC., SAN DIEGO, CA), FOLLOWED BY REFINEMENT WITH SIMULATED ANNEALING IN X-PLOR (T.F.HAVEL, A.T.BRUNGER). RESIDUE 1 IN ...詳細: MOLECULAR MODELS WERE OBTAINED BY DGII IMPLEMENTED IN INSIGHT II (BIOSYM INC., SAN DIEGO, CA), FOLLOWED BY REFINEMENT WITH SIMULATED ANNEALING IN X-PLOR (T.F.HAVEL, A.T.BRUNGER). RESIDUE 1 IN RESTRAINTS CORRESPONDS TO RESIDUE 35 IN DSX. TWO ZINC BINDING SITES ARE: C44,C47,H59,C63 AND H50,C68 C70, C73 EACH IN A TETRAHEDRAL PATTERN. (i.e., 10,13,25,29 and 16,34,36,39 in the coordinates). RESIDUE 35-40 AND 82-105 IN DEX ARE DISORDERED. Structures are giving for residues 35 to 86, corresponding to 1 to 52 numbered in the coordinates. NO MINOR CONFORMERS WERE DETECTED. | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 40 / 登録したコンフォーマーの数: 18 |