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- PDB-1lpb: THE 2.46 ANGSTROMS RESOLUTION STRUCTURE OF THE PANCREATIC LIPASE ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lpb | ||||||
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Title | THE 2.46 ANGSTROMS RESOLUTION STRUCTURE OF THE PANCREATIC LIPASE COLIPASE COMPLEX INHIBITED BY A C11 ALKYL PHOSPHONATE | ||||||
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![]() | HYDROLASE(CARBOXYLIC ESTERASE) | ||||||
Function / homology | ![]() positive regulation of triglyceride lipase activity / all-trans-retinyl-palmitate hydrolase, all-trans-retinol forming activity / Digestion of dietary lipid / Retinoid metabolism and transport / Digestion of dietary lipid / lipase binding / lipase activity / intestinal cholesterol absorption / triacylglycerol lipase / triacylglycerol lipase activity ...positive regulation of triglyceride lipase activity / all-trans-retinyl-palmitate hydrolase, all-trans-retinol forming activity / Digestion of dietary lipid / Retinoid metabolism and transport / Digestion of dietary lipid / lipase binding / lipase activity / intestinal cholesterol absorption / triacylglycerol lipase / triacylglycerol lipase activity / response to food / lipid catabolic process / Retinoid metabolism and transport / enzyme activator activity / digestion / lipid metabolic process / extracellular space / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Egloff, M.-P. / Van Tilbeurgh, H. / Cambillau, C. | ||||||
![]() | ![]() Title: The 2.46 A resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate. Authors: Egloff, M.P. / Marguet, F. / Buono, G. / Verger, R. / Cambillau, C. / van Tilbeurgh, H. #1: ![]() Title: Interfacial Activation of the Lipase-Procolipase Complex by Mixed Micelles Revealed by X-Ray Crystallography Authors: Van Tilbeurgh, H. / Egloff, M.-P. / Martinez, C. / Rugani, N. / Verger, R. / Cambillau, C. #2: ![]() Title: Structure of the Pancreatic Lipase-Procolipase Complex Authors: Van Tilbeurgh, H. / Sarda, L. / Verger, R. / Cambillau, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 151.3 KB | Display | ![]() |
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PDB format | ![]() | 123 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 638.7 KB | Display | ![]() |
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Full document | ![]() | 680.2 KB | Display | |
Data in XML | ![]() | 16.3 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO B 16 / 2: CIS PROLINE - PRO B 211 / 3: CIS PROLINE - PRO B 298 |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 10319.651 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Protein | Mass: 49573.562 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
-Sugars , 1 types, 5 molecules ![](data/chem/img/BOG.gif)
#3: Sugar | ChemComp-BOG / |
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-Non-polymers , 3 types, 287 molecules ![](data/chem/img/CA.gif)
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![](data/chem/img/HOH.gif)
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![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-CA / |
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#5: Chemical | ChemComp-MUP / |
#6: Water | ChemComp-HOH / |
-Details
Nonpolymer details | USE OF PARTIAL OCCUPANCY FOR THE INHIBITOR (REFINED OCCUPANCY) AND FOR BETA-OCTYLGLUCO |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.48 Å3/Da / Density % sol: 64.65 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.46 Å / Lowest resolution: 20 Å / Num. obs: 29845 / % possible obs: 92 % / Redundancy: 9.4 % / Num. measured all: 271893 / Rmerge(I) obs: 0.106 |
Reflection shell | *PLUS Highest resolution: 2.46 Å / Lowest resolution: 2.53 Å / % possible obs: 77.8 % / Redundancy: 3.9 % / Mean I/σ(I) obs: 84.9 |
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Processing
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Refinement | Resolution: 2.46→6 Å / Rfactor Rfree: 0.285 / Rfactor Rwork: 0.183 / Rfactor obs: 0.183 / σ(F): 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.46→6 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 27738 / Rfactor obs: 0.183 / Rfactor Rfree: 0.285 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.96 |