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- PDB-1loi: N-TERMINAL SPLICE REGION OF RAT C-AMP PHOSPHODIESTERASE, NMR, 7 S... -

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Basic information

Entry
Database: PDB / ID: 1loi
TitleN-TERMINAL SPLICE REGION OF RAT C-AMP PHOSPHODIESTERASE, NMR, 7 STRUCTURES
ComponentsCYCLIC 3',5'-AMP SPECIFIC PHOSPHODIESTERASE RD1
KeywordsHYDROLASE / C-AMP PHOSPHODIESTERASE
Function / homology
Function and homology information


DARPP-32 events / cellular response to xenobiotic stimulus => GO:0071466 / G alpha (s) signalling events / response to xenobiotic stimulus => GO:0009410 / 3',5'-cyclic-AMP phosphodiesterase / regulation of protein kinase A signaling / cAMP catabolic process / 3',5'-cyclic-AMP phosphodiesterase activity / regulation of cAMP-mediated signaling / cAMP binding ...DARPP-32 events / cellular response to xenobiotic stimulus => GO:0071466 / G alpha (s) signalling events / response to xenobiotic stimulus => GO:0009410 / 3',5'-cyclic-AMP phosphodiesterase / regulation of protein kinase A signaling / cAMP catabolic process / 3',5'-cyclic-AMP phosphodiesterase activity / regulation of cAMP-mediated signaling / cAMP binding / modulation of chemical synaptic transmission / Schaffer collateral - CA1 synapse / sensory perception of smell / perinuclear region of cytoplasm / signal transduction / membrane / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Phosphodiesterase 4 upstream conserved regions (UCR) / Phosphodiesterase 4 upstream conserved regions (UCR) / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain / 3'5'-cyclic nucleotide phosphodiesterase, conserved site / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain superfamily / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase domain signature. / 3'5'-cyclic nucleotide phosphodiesterase domain profile. / Metal dependent phosphohydrolases with conserved 'HD' motif. / HD/PDEase domain
Similarity search - Domain/homology
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodSOLUTION NMR
AuthorsSmith, K.J. / Scotland, G. / Beattie, J. / Trayer, I.P. / Houslay, M.D.
CitationJournal: J.Biol.Chem. / Year: 1996
Title: Determination of the structure of the N-terminal splice region of the cyclic AMP-specific phosphodiesterase RD1 (RNPDE4A1) by 1H NMR and identification of the membrane association domain using chimeric constructs.
Authors: Smith, K.J. / Scotland, G. / Beattie, J. / Trayer, I.P. / Houslay, M.D.
History
DepositionMay 21, 1996Processing site: BNL
Revision 1.0May 15, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: CYCLIC 3',5'-AMP SPECIFIC PHOSPHODIESTERASE RD1


Theoretical massNumber of molelcules
Total (without water)3,1211
Polymers3,1211
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)7 / -
Representative

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Components

#1: Protein/peptide CYCLIC 3',5'-AMP SPECIFIC PHOSPHODIESTERASE RD1 / RNPDE4A1A / RAT TYPE IV CYCLIC AMP SPECIFIC PHOSPHODIESTERASE / ISOFORM SUBFAMILY A / SPLICE VARIANT 1


Mass: 3120.667 Da / Num. of mol.: 1 / Fragment: RESIDUES 1 - 25
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Organ: BRAIN
References: UniProt: P54748, 3',5'-cyclic-nucleotide phosphodiesterase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: SOLVENT 50:50 TRIFLUOROETHANOL-D3:H2O.

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Sample preparation

Sample conditionspH: 6.0 / Temperature: 300 K
Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 7

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