Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
Remark 999
SEQUENCE Position 35 in U2 snRNA sequences from S. cerevisiae is a pseudouridine. This corresponds ...SEQUENCE Position 35 in U2 snRNA sequences from S. cerevisiae is a pseudouridine. This corresponds to position 5 on strand A, which is a uridine in 1LMV, and a pseudouridine in 1LPW.
分子量: 2912.775 Da / 分子数: 1 / 由来タイプ: 天然 詳細: unmodified U2 snRNA. Region of U2 snRNA that pairs with the intron branch site during spliceosome assembly (nucleotides 34-39 in yeast U2 snRNA). 由来: (天然) Saccharomyces cerevisiae (パン酵母)
#2: RNA鎖
5'-R(*UP*AP*CP*UP*AP*AP*CP*AP*CP*C)-3'
分子量: 3104.925 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Intron branch site sequence. Region of the pre-mRNA intron that contains the conserved branch site sequence, and pairs with U2 snRNA during spliceosome assembly. 由来: (天然) Saccharomyces cerevisiae (パン酵母)
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
2
2
2
2D NOESY
2
3
2
2D TOCSY
2
4
2
DQF-COSY
NMR実験の詳細
Text: This structure was determined using standard 2D homonuclear techniques. NOESY spectra were collected at many different mixing times to assess the buildup rate of each NOE, thereby resolving ...Text: This structure was determined using standard 2D homonuclear techniques. NOESY spectra were collected at many different mixing times to assess the buildup rate of each NOE, thereby resolving some of the spectral overlap.
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1mM unmodified U2 snNRA - intron branch site duplex, 10mM sodium phosphate, 0.1mM EDTA
90% H2O/10% D2O
2
1mM unmodified U2 snNRA - intron branch site duplex, 10mM sodium phosphate, 0.1mM EDTA