[English] 日本語
![](img/lk-miru.gif)
- PDB-1lln: 1.6A CRYSTAL STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN-III (PAP-III... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1lln | ||||||
---|---|---|---|---|---|---|---|
Title | 1.6A CRYSTAL STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN-III (PAP-III) WITH METHYLATED LYSINES | ||||||
![]() | Antiviral Protein 3 | ||||||
![]() | HYDROLASE / POKEWEED ANTIVIRAL PROTEIN / RIBOSOME INACTIVATING PROTEIN / POLYNUCLEOTIDE:ADENOSINE | ||||||
Function / homology | ![]() rRNA N-glycosylase / rRNA N-glycosylase activity / toxin activity / defense response to virus / negative regulation of translation Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kurinov, I.V. / Uckun, F.M. | ||||||
![]() | ![]() Title: High resolution X-ray structure of potent anti-HIV pokeweed antiviral protein-III Authors: Kurinov, I.V. / Uckun, F.M. #1: ![]() Title: X-Ray Crystallographic Analysis of the Structural Basis for the Interactions of Pokeweed Antiviral Protein with its Active Site Inhibitor and Ribosomal RNA Substrate Analogs Authors: Kurinov, I.V. / Myers, D.E. / Irvin, J.D. / Uckun, F.M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 65 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 53.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 29683.041 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: late summer leaves / Source: (natural) ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 48.73 % | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 9.5 Details: 30% PEG400, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K | ||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 90 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 8, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. all: 38088 / Num. obs: 37018 / % possible obs: 97.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.4 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 23.9 |
Reflection shell | Resolution: 1.6→1.66 Å / Rmerge(I) obs: 0.132 / % possible all: 96.9 |
Reflection | *PLUS Highest resolution: 1.6 Å |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→7 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.6 Å / Rfactor Rwork: 0.203 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
|