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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1l6e | ||||||
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タイトル | Solution structure of the docking and dimerization domain of protein kinase A II-alpha (RIIalpha D/D). Alternatively called the N-terminal dimerization domain of the regulatory subunit of protein kinase A. | ||||||
![]() | cAMP-dependent protein kinase Type II-alpha regulatory chain | ||||||
![]() | TRANSFERASE / Four-helix bundle / helix-loop-helix / regulatory subunit / dimerization / docking / anchoring | ||||||
機能・相同性 | ![]() PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / GPER1 signaling / Hedgehog 'off' state / Factors involved in megakaryocyte development and platelet production / cAMP-dependent protein kinase regulator activity / cAMP-dependent protein kinase inhibitor activity ...PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / GPER1 signaling / Hedgehog 'off' state / Factors involved in megakaryocyte development and platelet production / cAMP-dependent protein kinase regulator activity / cAMP-dependent protein kinase inhibitor activity / beta-2 adrenergic receptor binding / cAMP-dependent protein kinase complex / small molecule binding / protein kinase A catalytic subunit binding / cAMP binding / T-tubule / regulation of protein phosphorylation / modulation of chemical synaptic transmission / protein domain specific binding / glutamatergic synapse / synapse / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / identical protein binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / Hybrid distance geometry-dynamical simulated annealing, refinement protocol for monomer structure determination, with 457 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 sequential, |i-j|=1; 95 medium range, 1<|i-j|<5; 41 long range, |i-j|>4), 19 distance restraints representing hydrogen bonds (entered as 2 distances each), 25 phi-, 5 chi1-torsion angle restraints. Molecular dynamical simulated annealing protocol for dimer structure determination, using 505 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 sequential, |i-j|=1; 95 medium range, 1<|i-j|<5; 25 long range, |i-j|>4; 38 inter-molecular; 26 ambiguous), 19 distance restraints representing hydrogen bonds (entered as 2 distances each), 25 phi-, 5 chi1-torsion angle restraints. | ||||||
![]() | Morikis, D. / Roy, M. / Newlon, M.G. / Scott, J.D. / Jennings, P.A. | ||||||
![]() | ![]() タイトル: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIalpha 著者: Morikis, D. / Roy, M. / Newlon, M.G. / Scott, J.D. / Jennings, P.A. #1: ![]() タイトル: The molecular basis for protein kinase A anchoring revealed by solution NMR. 著者: Newlon, M.G. / Roy, M. / Morikis, D. / Hausken, Z.E. / Coghlan, V. / Scott, J.D. / Jennings, P.A. #2: ![]() タイトル: The A-kinase anchoring domain of type II-alpha cAMP-dependent protein kinase is highly helical. 著者: Newlon, M.G. / Roy, M. / Hausken, Z.E. / Scott, J.D. / Jennings, P.A. | ||||||
履歴 |
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Remark 999 | SEQUENCE THE FIRST 3 RESIDUES ARE DIFFERENT DUE TO RECOMBINANT EXPRESSION AND PROTEOLYTIC CLEAVAGE. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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PDBx/mmCIF形式 | ![]() | 709.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 595.7 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質・ペプチド | 分子量: 5398.181 Da / 分子数: 2 / 断片: N-terminal docking and dimerization domain / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験の詳細 | Text: This structure was determined using standard homonuclear, heteronuclear, and triple resonance spectroscopy, and 3D 13C-edited(w2)-12C-filtered(w1)/13C-filtered(w3) NOESY. |
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試料調製
詳細 |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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解析
NMR software |
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精密化 | 手法: Hybrid distance geometry-dynamical simulated annealing, refinement protocol for monomer structure determination, with 457 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 ...手法: Hybrid distance geometry-dynamical simulated annealing, refinement protocol for monomer structure determination, with 457 NOE-derived distance restraints (185 intra-residue, i-j=0; 136 sequential, |i-j|=1; 95 medium range, 1 ソフトェア番号: 1 詳細: Filtered NOESY spectrum on a 50% unlabeled-50% 13C-15N-labeled sample was used to obtain inter-molecular NOE contacts of the homodimer. Other NOEs were classified as intra-molecular and ambiguous. | ||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations,structures with the lowest energy 計算したコンフォーマーの数: 49 / 登録したコンフォーマーの数: 24 |