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Open data
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Basic information
Entry | Database: PDB / ID: 1l3d | ||||||
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Title | Low Resolution Crystal Structure of a Viral RNA Pseudoknot | ||||||
![]() | RNA pseudoknot | ||||||
![]() | RNA / Pseudoknot / frameshifting / viral RNA / flexibility | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Egli, M. / Minasov, G. / Su, L. / Rich, A. | ||||||
![]() | ![]() Title: Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution. Authors: Egli, M. / Minasov, G. / Su, L. / Rich, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 25.8 KB | Display | ![]() |
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PDB format | ![]() | 18.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 380.7 KB | Display | ![]() |
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Full document | ![]() | 382.7 KB | Display | |
Data in XML | ![]() | 3.1 KB | Display | |
Data in CIF | ![]() | 3.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1l2xC ![]() 437dS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: RNA chain | Mass: 9245.484 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: RNA SEQUENCE TAKEN FROM BEET WESTERN YELLOW VIRUS |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.65 Å3/Da / Density % sol: 78.22 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5 Details: ammonium sulfate, sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K | ||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 4 ℃ | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 17, 1997 / Details: mirrors |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→30 Å / Num. all: 5034 / Num. obs: 5034 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 9.2 % / Biso Wilson estimate: 63.1 Å2 / Rmerge(I) obs: 0.054 / Net I/σ(I): 39.5 |
Reflection shell | Resolution: 2.85→2.95 Å / Redundancy: 8.9 % / Rmerge(I) obs: 0.286 / Mean I/σ(I) obs: 9.3 / Num. unique all: 486 / % possible all: 100 |
Reflection | *PLUS % possible obs: 100 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 437D Resolution: 2.85→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 Stereochemistry target values: G. Parkinson et al., Acta Cryst. (1996) D52, 57-64 Details: Crystallographic conjugate gradient minimization refinement using maximum likelihood target for amplitudes
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Displacement parameters | Biso mean: 43.3 Å2
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Refinement step | Cycle: LAST / Resolution: 2.85→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.85→2.98 Å
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Refinement | *PLUS % reflection Rfree: 10 % / Rfactor obs: 0.271 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1 | |||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor obs: 0.427 |