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- PDB-1ku3: Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subun... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ku3 | ||||||
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Title | Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment, Region 4 | ||||||
![]() | sigma factor sigA | ||||||
![]() | TRANSCRIPTION / helix-turn-helix | ||||||
Function / homology | ![]() sigma factor activity / DNA-templated transcription initiation / DNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Campbell, E.A. / Muzzin, O. / Chlenov, M. / Sun, J.L. / Olson, C.A. / Weinman, O. / Trester-Zedlitz, M.L. / Darst, S.A. | ||||||
![]() | ![]() Title: Structure of the bacterial RNA polymerase promoter specificity sigma subunit. Authors: Campbell, E.A. / Muzzin, O. / Chlenov, M. / Sun, J.L. / Olson, C.A. / Weinman, O. / Trester-Zedlitz, M.L. / Darst, S.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 23.7 KB | Display | ![]() |
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PDB format | ![]() | 15.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 364.8 KB | Display | ![]() |
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Full document | ![]() | 367 KB | Display | |
Data in XML | ![]() | 2.6 KB | Display | |
Data in CIF | ![]() | 3.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8737.127 Da / Num. of mol.: 1 / Fragment: region 4 (residues 366-438) / Mutation: L386M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.75 Å3/Da / Density % sol: 29.66 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6 Details: MES, ammonium sulfate, magnesium chloride, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22.5 ℃ / pH: 6 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Sep 14, 2001 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. all: 6121 / Num. obs: 5999 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.3 % / Rmerge(I) obs: 0.047 / Net I/σ(I): 30 |
Reflection shell | Resolution: 1.8→1.85 Å / Rmerge(I) obs: 0.237 / Mean I/σ(I) obs: 6.7 / % possible all: 99.3 |
Reflection | *PLUS Lowest resolution: 30 Å / % possible obs: 98 % / Num. measured all: 49941 / Rmerge(I) obs: 0.047 |
Reflection shell | *PLUS % possible obs: 99.3 % / Rmerge(I) obs: 0.237 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.8→30 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 30 Å / % reflection Rfree: 5 % / Rfactor obs: 0.218 / Rfactor Rfree: 0.252 / Rfactor Rwork: 0.218 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |