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Open data
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Basic information
Entry | Database: PDB / ID: 1koz | ||||||
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Title | SOLUTION STRUCTURE OF OMEGA-GRAMMOTOXIN SIA | ||||||
![]() | Voltage-dependent Channel Inhibitor | ||||||
![]() | TOXIN / CYSTINE KNOT | ||||||
Function / homology | Huwentoxin-1 family / Ion channel inhibitory toxin / potassium channel inhibitor activity / : / calcium channel inhibitor activity / toxin activity / extracellular region / Omega-theraphotoxin-Gr1a![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Takeuchi, K. / Park, E.J. / Lee, C.W. / Kim, J.I. / Takahashi, H. / Swartz, K.J. / Shimada, I. | ||||||
![]() | ![]() Title: Solution structure of omega-grammotoxin SIA, a gating modifier of P/Q and N-type Ca(2+) channel. Authors: Takeuchi, K. / Park, E. / Lee, C. / Kim, J. / Takahashi, H. / Swartz, K. / Shimada, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 217.7 KB | Display | ![]() |
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PDB format | ![]() | 186.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 340.9 KB | Display | ![]() |
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Full document | ![]() | 471.7 KB | Display | |
Data in XML | ![]() | 23.1 KB | Display | |
Data in CIF | ![]() | 34.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 4122.778 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: chemically synthesized by a solid-phase methodology References: UniProt: P60590 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES. |
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Sample preparation
Details | Contents: 2.4mM Grammotoxin NA; 90% H2O, 10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 0 / pH: 3.5 / Pressure: 1 atm / Temperature: 283 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: Additional comments about the NMR refinement can be placed here, e.g. the structures are based on a total of 561 restraints, 536 are NOE-derived distance constraints, 20 dihedral angle ...Details: Additional comments about the NMR refinement can be placed here, e.g. the structures are based on a total of 561 restraints, 536 are NOE-derived distance constraints, 20 dihedral angle restraints,5 distance restraints from hydrogen bonds. | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |