[English] 日本語
![](img/lk-miru.gif)
- PDB-1koa: TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINAS... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1koa | ||||||
---|---|---|---|---|---|---|---|
Title | TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS | ||||||
![]() | TWITCHIN | ||||||
![]() | KINASE / TWITCHIN / INTRASTERIC REGULATION | ||||||
Function / homology | ![]() positive regulation of locomotion / positive regulation of sarcomere organization / positive regulation of striated muscle contraction / negative regulation of cell division / behavioral response to nicotine / A band / sarcomere organization / adult locomotory behavior / non-specific serine/threonine protein kinase / calmodulin binding ...positive regulation of locomotion / positive regulation of sarcomere organization / positive regulation of striated muscle contraction / negative regulation of cell division / behavioral response to nicotine / A band / sarcomere organization / adult locomotory behavior / non-specific serine/threonine protein kinase / calmodulin binding / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / protein kinase binding / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kobe, B. / Heierhorst, J. / Feil, S.C. / Parker, M.W. / Benian, G.M. / Weiss, K.R. / Kemp, B.E. | ||||||
![]() | ![]() Title: Giant protein kinases: domain interactions and structural basis of autoregulation. Authors: Kobe, B. / Heierhorst, J. / Feil, S.C. / Parker, M.W. / Benian, G.M. / Weiss, K.R. / Kemp, B.E. #1: ![]() Title: Ca2+/S100 Regulation of Giant Protein Kinases Authors: Heierhorst, J. / Kobe, B. / Feil, S.C. / Parker, M.W. / Benian, G.M. / Weiss, K.R. / Kemp, B.E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 98.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 73.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 449.2 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 28.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 56426.305 Da / Num. of mol.: 1 / Fragment: KINASE FRAGMENT Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 5.52 Å3/Da / Density % sol: 77.71 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 7.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
---|---|
Detector | Detector: IMAGE PLATE / Date: 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Num. obs: 17405 / % possible obs: 83.4 % / Observed criterion σ(I): -3 / Redundancy: 4.8 % / Biso Wilson estimate: 100 Å2 / Rmerge(I) obs: 0.157 |
Reflection | *PLUS Highest resolution: 3.3 Å / Lowest resolution: 3.7 Å / Num. measured all: 39131 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 3.3→40 Å / σ(F): 0 Details: B-FACTORS WERE REFINED AS 2 GROUPS PER RESIDUE (MAIN-CHAIN ATOMS, SIDE-CHAIN ATOMS).
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 88.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.3→40 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.3425 / Rfactor Rwork: 0.2719 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |