+Open data
-Basic information
Entry | Database: PDB / ID: 1k8h | ||||||
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Title | NMR Structure of Small Protein B (SmpB) from Aquifex aeolicus | ||||||
Components | Small protein B | ||||||
Keywords | RNA BINDING PROTEIN / SmpB / SsrA associated protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Aquifex aeolicus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Dong, G. / Hoffman, D.W. | ||||||
Citation | Journal: EMBO J. / Year: 2002 Title: Structure of small protein B: the protein component of the tmRNA-SmpB system for ribosome rescue. Authors: Dong, G. / Nowakowski, J. / Hoffman, D.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k8h.cif.gz | 445 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k8h.ent.gz | 368.2 KB | Display | PDB format |
PDBx/mmJSON format | 1k8h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1k8h_validation.pdf.gz | 344.3 KB | Display | wwPDB validaton report |
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Full document | 1k8h_full_validation.pdf.gz | 457.8 KB | Display | |
Data in XML | 1k8h_validation.xml.gz | 34.2 KB | Display | |
Data in CIF | 1k8h_validation.cif.gz | 44.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k8/1k8h ftp://data.pdbj.org/pub/pdb/validation_reports/k8/1k8h | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15523.292 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aquifex aeolicus (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: O66640 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was solved using standard 2D and 3D heteronuclear NMR techniques. |
-Sample preparation
Details | Contents: 1 mM SmpB / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100 mM / pH: 6.0 / Pressure: ambient / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software | Name: CNS / Version: 1 / Developer: Brunger / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 |