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Open data
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Basic information
Entry | Database: PDB / ID: 1k4a | ||||||||||||||||||
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Title | STRUCTURE OF AGAA RNA TETRALOOP, NMR, 20 STRUCTURES | ||||||||||||||||||
![]() | 5'-R(*![]() RNA / Ribonucleic Acid / Tetraloop | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / distance geometry simulated annealing | ![]() Wu, H. / Yang, P.K. / Butcher, S.E. / Kang, S. / Chanfreau, G. / Feigon, J. | ![]() ![]() Title: A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III. Authors: Wu, H. / Yang, P.K. / Butcher, S.E. / Kang, S. / Chanfreau, G. / Feigon, J. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 175 KB | Display | ![]() |
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PDB format | ![]() | 143.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 323.5 KB | Display | ![]() |
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Full document | ![]() | 441.3 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 14.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 4509.771 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: An RNA Hairpin with AGAA Tetraloop, the primary recognition site for S. cerevisiae RNase III. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 326 restraints, 300 are NOE-derived distance restraints, 54 dihedral angle restraints. Additional 26 distance restraints from hydrogen bonds are used in the stem region. | ||||||||||||||||
NMR representative | Selection criteria: this is the lowest energy structure | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |