Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
Remark 999
Sequence The last four amino acid segment IEGR, corresponding to the FactorXa recognition site used ...Sequence The last four amino acid segment IEGR, corresponding to the FactorXa recognition site used to remove the histidine tag, was engineered at the C-terminus of the construct.
Remark 600
HETEROGEN COPPER (I) IS COORDINATED BY CYS 13 AND CYS 16.
Text: The structure was determined using heteronuclear technique
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試料調製
詳細
Solution-ID
内容
溶媒系
1
Sample 1: 2mM Copper (I) loaded-Bacillus subtilis CopZ, 15N labelled. DTT was used as reductant. Sample 2: 2mM Copper (I) loaded-Bacillus subtilis CopZ, not labelled. DTT was used as reductant.