+Open data
-Basic information
Entry | Database: PDB / ID: 1ju8 | ||||||
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Title | Solution structure of Leginsulin, a plant hormon | ||||||
Components | Leginsulin | ||||||
Keywords | HORMONE/GROWTH FACTOR / T-knot / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | Albumin I / Albumin I chain b / Albumin I chain a / Albumin I chain a / nutrient reservoir activity / : / toxin activity / Albumin-1 Function and homology information | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing, molecular dynamics | ||||||
Authors | Yamazaki, T. / Takaoka, M. / Katoh, E. / Hanada, K. / Sakita, M. / Sakata, K. / Nishiuchi, Y. / Hirano, H. | ||||||
Citation | Journal: EUR.J.BIOCHEM. / Year: 2003 Title: A possible physiological function and the tertiary structure of a 4-kDa peptide in legumes Authors: Yamazaki, T. / Takaoka, M. / Katoh, E. / Hanada, K. / Sakita, M. / Sakata, K. / Nishiuchi, Y. / Hirano, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ju8.cif.gz | 17.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ju8.ent.gz | 12.8 KB | Display | PDB format |
PDBx/mmJSON format | 1ju8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ju/1ju8 ftp://data.pdbj.org/pub/pdb/validation_reports/ju/1ju8 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3929.575 Da / Num. of mol.: 1 / Fragment: residues 1-37 / Source method: obtained synthetically Details: This sequence occurs naturally in legumes of Glycine max. References: UniProt: Q39837 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 4mM leginsulin non-labeled; 70% H2O, 30% CD3COOD / Solvent system: 70% H2O, 30% CD3COOD |
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Sample conditions | Ionic strength: 0.0 / pH: 1.8 / Pressure: amient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 750 MHz |
-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing, molecular dynamics Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 15 structures with the lowest energy. Conformers calculated total number: 100 / Conformers submitted total number: 1 |