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- PDB-1jqg: Crystal Structure of the Carboxypeptidase A from Helicoverpa Armigera -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jqg | ||||||
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Title | Crystal Structure of the Carboxypeptidase A from Helicoverpa Armigera | ||||||
![]() | carboxypeptidase A | ||||||
![]() | HYDROLASE / PRO-PROTEIN | ||||||
Function / homology | ![]() carboxypeptidase A / metallocarboxypeptidase activity / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Estebanez-Perpina, E. / Bayes, A. / Vendrell, J. / Jongsma, M.A. / Bown, D.P. / Gatehouse, J.A. / Huber, R. / Bode, W. / Aviles, F.X. / Reverter, D. | ||||||
![]() | ![]() Title: Crystal structure of a novel mid-gut procarboxypeptidase from the cotton pest Helicoverpa armigera. Authors: Estebanez-Perpina, E. / Bayes, A. / Vendrell, J. / Jongsma, M.A. / Bown, D.P. / Gatehouse, J.A. / Huber, R. / Bode, W. / Aviles, F.X. / Reverter, D. #1: ![]() Title: Midgut carboxypeptidase from Helicoverpa armigera (Lepidoptera: Noctuidae) larvae: enzyme characterisation, cDNA cloning and expression. Authors: Bown, D.P. / Wilkinson, H.S. / Gatehouse, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.1 KB | Display | ![]() |
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PDB format | ![]() | 72.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1ayeS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48539.023 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42.05 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: PEG 8000, Na-Acetate, Na-Cacodylate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: unknown / Details: used macroseeding | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 16, 2000 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→10 Å / Num. all: 230224 / Num. obs: 21449 / % possible obs: 93.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 15.6 |
Reflection shell | Resolution: 2.5→2.6 Å / Rmerge(I) obs: 0.034 / Mean I/σ(I) obs: 2.5 / % possible all: 93 |
Reflection | *PLUS Lowest resolution: 12 Å / Num. measured all: 230224 |
Reflection shell | *PLUS % possible obs: 93 % / Rmerge(I) obs: 0.344 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AYE Resolution: 2.5→10 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.6 Å
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Refinement | *PLUS Lowest resolution: 12 Å / % reflection Rfree: 7 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.5 |