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- PDB-1jl2: Crystal structure of TCEO RNase H-a chimera combining the folding... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jl2 | ||||||
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Title | Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H | ||||||
![]() | Chimera of Ribonuclease HI, Ribonuclease H | ||||||
![]() | HYDROLASE / mixed alpha-beta protein | ||||||
Function / homology | ![]() DNA replication, removal of RNA primer / ribonuclease H / RNA-DNA hybrid ribonuclease activity / endonuclease activity / nucleic acid binding / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Robic, S. / Berger, J.M. / Marqusee, S. | ||||||
![]() | ![]() Title: Contributions of folding cores to the thermostabilities of two ribonucleases H. Authors: Robic, S. / Berger, J.M. / Marqusee, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 123.9 KB | Display | ![]() |
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PDB format | ![]() | 97.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 389.7 KB | Display | ![]() |
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Full document | ![]() | 402.1 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 21.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1f21S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17600.957 Da / Num. of mol.: 4 Fragment: P0A7Y4 residues 1-42, 123-155, P29253 residues 47-127 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() Genus: Escherichia, Thermus / Species: , / Strain: K12, HB8 / ATCC 27634 / DSM 579 Gene: rnhA, dasF, herA, rnh, sdrA, b0214, JW0204, rnhA, TTHA1556 Plasmid: pSR202 / Species (production host): Escherichia coli / Production host: ![]() ![]() References: UniProt: P0A7Y4, UniProt: P29253, ribonuclease H #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.59 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 50 mM Tris, pH 8.0, 18% PEG600, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 28, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→20 Å / Num. all: 64081 / Num. obs: 62607 / % possible obs: 97.7 % / Rmerge(I) obs: 0.061 |
Reflection shell | Resolution: 1.76→1.83 Å / Rmerge(I) obs: 0.277 / % possible all: 95.7 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 5255 / Num. measured all: 62607 |
Reflection shell | *PLUS % possible obs: 95.7 % / Num. unique obs: 189 / Num. measured obs: 6102 / Mean I/σ(I) obs: 2.52 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1f21 Resolution: 1.76→20 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 1.76→20 Å
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / % reflection Rfree: 10 % / Rfactor obs: 0.249 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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