+Open data
-Basic information
Entry | Database: PDB / ID: 1jkz | ||||||
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Title | NMR Solution Structure of Pisum sativum defensin 1 (Psd1) | ||||||
Components | DEFENSE-RELATED PEPTIDE 1 | ||||||
Keywords | ANTIFUNGAL PROTEIN / PLANT DEFENSIN / CYS-RICH / ANTIFUNGAL | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pisum sativum (garden pea) | ||||||
Method | SOLUTION NMR / THE STRUCTURE WAS CALCULATED USING TORSIONAL SIMULATED ANNEALING, THEN MINIMIZED | ||||||
Authors | Almeida, M.S. / Cabral, K.M.S. / Kurtenbach, E. / Almeida, F.C.L. / Valente, A.P. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2002 Title: Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action. Authors: Almeida, M.S. / Cabral, K.M. / Kurtenbach, E. / Almeida, F.C. / Valente, A.P. #1: Journal: To be Published Title: cDNA CLONING AND HETEROLOGOUS EXPRESSION OF FUNCTIONAL CYS-RICH ANTIFUNGAL PROTEIN PSD1 IN THE YEAST PICHIA PASTORIS Authors: ALMEIDA, M.S. / CABRAL, K.M.S. / MEDEIROS, L.N. / VALENTE, A.P. / ALMEIDA, F.C.L. / KURTENBACH, E. #2: Journal: Arch.Biochem.Biophys. / Year: 2000 Title: Characterization of two novel defense peptides from pea (Pisum sativum) seeds Authors: ALMEIDA, M.S. / CABRAL, K.M. / ZINGALI, R.B. / KURTENBACH, E. | ||||||
History |
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Remark 999 | SEQUENCE An appropriate sequence database reference was not available at the time of processing. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1jkz.cif.gz | 266.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1jkz.ent.gz | 231.4 KB | Display | PDB format |
PDBx/mmJSON format | 1jkz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1jkz_validation.pdf.gz | 342.6 KB | Display | wwPDB validaton report |
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Full document | 1jkz_full_validation.pdf.gz | 438.6 KB | Display | |
Data in XML | 1jkz_validation.xml.gz | 19.1 KB | Display | |
Data in CIF | 1jkz_validation.cif.gz | 30.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jk/1jkz ftp://data.pdbj.org/pub/pdb/validation_reports/jk/1jkz | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 5217.923 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: ISOLATED FROM PEA SEEDS / Source: (natural) Pisum sativum (garden pea) / References: UniProt: P81929*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.8 mM PSD1 / Solvent system: 10 mM SODIUM PHOSPHATE BUFFER pH 4 |
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Sample conditions | Ionic strength: 10 mM sodium phosphate / pH: 4 / Pressure: AMBIENT / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: THE STRUCTURE WAS CALCULATED USING TORSIONAL SIMULATED ANNEALING, THEN MINIMIZED Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |