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Yorodumi- PDB-1j86: HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), MONOCLINIC ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1j86 | |||||||||
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Title | HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), MONOCLINIC CRYSTAL FORM 2 | |||||||||
Components | HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR ALPHA-SUBUNIT | |||||||||
Keywords | IMMUNE SYSTEM / Fc Receptor / IgE receptor / Glycoprotein | |||||||||
Function / homology | Function and homology information high-affinity IgE receptor activity / type I hypersensitivity / eosinophil degranulation / IgE binding / type 2 immune response / Fc epsilon receptor (FCERI) signaling / mast cell degranulation / regulation of immune response / Role of LAT2/NTAL/LAB on calcium mobilization / FCERI mediated Ca+2 mobilization ...high-affinity IgE receptor activity / type I hypersensitivity / eosinophil degranulation / IgE binding / type 2 immune response / Fc epsilon receptor (FCERI) signaling / mast cell degranulation / regulation of immune response / Role of LAT2/NTAL/LAB on calcium mobilization / FCERI mediated Ca+2 mobilization / FCERI mediated MAPK activation / FCERI mediated NF-kB activation / transmembrane signaling receptor activity / cell surface receptor signaling pathway / cell surface / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | |||||||||
Authors | Garman, S.C. / Sechi, S. / Kinet, J.P. / Jardetzky, T.S. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: The analysis of the human high affinity IgE receptor Fc epsilon Ri alpha from multiple crystal forms. Authors: Garman, S.C. / Sechi, S. / Kinet, J.P. / Jardetzky, T.S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1j86.cif.gz | 92.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1j86.ent.gz | 71.2 KB | Display | PDB format |
PDBx/mmJSON format | 1j86.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j8/1j86 ftp://data.pdbj.org/pub/pdb/validation_reports/j8/1j86 | HTTPS FTP |
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-Related structure data
Related structure data | 1j87C 1j88C 1j89C 1f2qS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | The biological assembly is a protein monomer with attached carbohydrate |
-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 20462.738 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR FRAGMENT Source method: isolated from a genetically manipulated source Details: GLYCOSYLATED PROTEIN, CHAIN A BY SUGARS C, CHAIN B BY SUGARS D Source: (gene. exp.) Homo sapiens (human) / Plasmid: PVL1392 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P12319 |
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-Sugars , 6 types, 10 molecules
#2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #4: Polysaccharide | alpha-D-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta- ...alpha-D-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #5: Polysaccharide | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #6: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-D-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-D-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #7: Sugar | ChemComp-NAG / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.33 Å3/Da / Density % sol: 68 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 4000, HEPES, ISOPROPANOL. pH 7.5, VAPOR DIFFUSION, HANGING DROP at 293K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 23. ℃ / pH: 8.5 / Details: Garman, S.C., (2000) Nature, 406, 259. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 5ID-B / Wavelength: 1.005 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 2, 1997 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.005 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→40 Å / Num. all: 11640 / Num. obs: 11640 / % possible obs: 99.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 70.8 Å2 / Rmerge(I) obs: 0.097 / Net I/σ(I): 15.7 |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.436 / % possible all: 100 |
Reflection | *PLUS Lowest resolution: 40 Å / Num. measured all: 47238 |
Reflection shell | *PLUS % possible obs: 100 % / Mean I/σ(I) obs: 3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1F2Q Resolution: 3.2→22.65 Å / Rfactor Rfree error: 0.011 / Data cutoff high absF: 1431976.63 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: 300 kcal/mol/A^2 NCS restraints applied to all protein atoms except those in crystal contacts, in flexible loops, or with attached carbohydrate.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 10 Å2 / ksol: 0.214 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 67.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.2→22.65 Å
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Refine LS restraints |
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Refine LS restraints NCS | Rms dev position: 0.04 Å / Weight Biso : 2 / Weight position: 300 | ||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.2→3.4 Å / Rfactor Rfree error: 0.034 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 5.3 % | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 67.5 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.328 / % reflection Rfree: 4.8 % / Rfactor Rwork: 0.305 |