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Yorodumi- PDB-1ix2: Crystal Structure of Selenomethionine PcoC, a Copper Resistance P... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ix2 | ||||||
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Title | Crystal Structure of Selenomethionine PcoC, a Copper Resistance Protein from Escherichia coli | ||||||
Components | PcoC copper resistance protein | ||||||
Keywords | METAL BINDING PROTEIN / BETA BARREL / POLYMETHIONINE CLUSTER | ||||||
Function / homology | Function and homology information copper ion transport / response to copper ion / periplasmic space / copper ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.55 Å | ||||||
Authors | Wernimont, A.K. / Huffman, D.L. / Finney, L.A. / Demeler, B. / O'Halloran, T.V. / Rosenzweig, A.C. | ||||||
Citation | Journal: J.BIOL.INORG.CHEM. / Year: 2003 Title: Crystal structure and dimerization equilibria of PcoC, a methionine-rich copper resistance protein from Escherichia coli Authors: Wernimont, A.K. / Huffman, D.L. / Finney, L.A. / Demeler, B. / O'Halloran, T.V. / Rosenzweig, A.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ix2.cif.gz | 53.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ix2.ent.gz | 39.6 KB | Display | PDB format |
PDBx/mmJSON format | 1ix2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ix2_validation.pdf.gz | 429.3 KB | Display | wwPDB validaton report |
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Full document | 1ix2_full_validation.pdf.gz | 432.2 KB | Display | |
Data in XML | 1ix2_validation.xml.gz | 12 KB | Display | |
Data in CIF | 1ix2_validation.cif.gz | 16.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ix/1ix2 ftp://data.pdbj.org/pub/pdb/validation_reports/ix/1ix2 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11298.170 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: plasmid pRJ1004 / Plasmid: pDLHII265 / Production host: Escherichia coli (E. coli) / Strain (production host): B834(DE3) / References: UniProt: Q47454 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.48 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: SODIUM CITRATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 1.55→30 Å / Num. all: 30839 / Num. obs: 30655 / % possible obs: 99.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 4 % / Biso Wilson estimate: 20.1 Å2 / Rmerge(I) obs: 0.081 / Rsym value: 0.067 / Net I/σ(I): 8.5 | |||||||||||||||
Reflection shell | Resolution: 1.55→1.61 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.302 / Mean I/σ(I) obs: 1.8 / Num. unique all: 3031 / Rsym value: 0.312 / % possible all: 99.4 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.55→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 22.94 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.55→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.55→1.62 Å / Rfactor Rfree error: 0.016
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