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- PDB-1irk: CRYSTAL STRUCTURE OF THE TYROSINE KINASE DOMAIN OF THE HUMAN INSU... -
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Basic information
Entry | Database: PDB / ID: 1irk | ||||||
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Title | CRYSTAL STRUCTURE OF THE TYROSINE KINASE DOMAIN OF THE HUMAN INSULIN RECEPTOR | ||||||
![]() | INSULIN RECEPTOR TYROSINE KINASE DOMAIN | ||||||
![]() | TRANSFERASE (PHOSPHOTRANSFERASE) | ||||||
Function / homology | ![]() regulation of female gonad development / positive regulation of meiotic cell cycle / insulin-like growth factor II binding / positive regulation of developmental growth / male sex determination / exocrine pancreas development / insulin receptor complex / insulin-like growth factor I binding / insulin receptor activity / positive regulation of protein-containing complex disassembly ...regulation of female gonad development / positive regulation of meiotic cell cycle / insulin-like growth factor II binding / positive regulation of developmental growth / male sex determination / exocrine pancreas development / insulin receptor complex / insulin-like growth factor I binding / insulin receptor activity / positive regulation of protein-containing complex disassembly / cargo receptor activity / dendritic spine maintenance / insulin binding / PTB domain binding / adrenal gland development / Signaling by Insulin receptor / IRS activation / activation of protein kinase activity / neuronal cell body membrane / amyloid-beta clearance / positive regulation of respiratory burst / positive regulation of receptor internalization / regulation of embryonic development / insulin receptor substrate binding / transport across blood-brain barrier / positive regulation of glycogen biosynthetic process / epidermis development / Signal attenuation / phosphatidylinositol 3-kinase binding / heart morphogenesis / Insulin receptor recycling / insulin-like growth factor receptor binding / dendrite membrane / neuron projection maintenance / activation of protein kinase B activity / positive regulation of glycolytic process / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / receptor-mediated endocytosis / learning / caveola / positive regulation of glucose import / positive regulation of MAP kinase activity / receptor protein-tyrosine kinase / receptor internalization / memory / cellular response to growth factor stimulus / peptidyl-tyrosine phosphorylation / cellular response to insulin stimulus / male gonad development / positive regulation of nitric oxide biosynthetic process / late endosome / insulin receptor signaling pathway / glucose homeostasis / amyloid-beta binding / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protein tyrosine kinase activity / positive regulation of MAPK cascade / protein autophosphorylation / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor complex / lysosome / endosome membrane / positive regulation of cell migration / symbiont entry into host cell / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / protein domain specific binding / external side of plasma membrane / axon / protein phosphorylation / positive regulation of cell population proliferation / regulation of DNA-templated transcription / protein-containing complex binding / GTP binding / positive regulation of DNA-templated transcription / extracellular exosome / ATP binding / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Hubbard, S.R. / Wei, L. / Ellis, L. / Hendrickson, W.A. | ||||||
![]() | ![]() Title: Crystal structure of the tyrosine kinase domain of the human insulin receptor. Authors: Hubbard, S.R. / Wei, L. / Ellis, L. / Hendrickson, W.A. #1: ![]() Title: Expression, Characterization and Crystallization of the Catalytic Core of the Human Insulin Receptor Protein Tyrosine Kinase Domain Authors: Wei, L. / Hubbard, S.R. / Hendrickson, W.A. / Ellis, L. #2: ![]() Title: The Human Insulin Receptor Cdna: The Structural Basis for Hormone-Activated Transmembrane Signalling Authors: Ebina, Y. / Ellis, L. / Jarnagin, K. / Edery, M. / Graf, L. / Clauser, E. / Ou, J.-H. / Masiarz, F. / Kan, Y.W. / Goldfine, I.D. / Roth, R.A. / Rutter, W.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 77.2 KB | Display | ![]() |
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PDB format | ![]() | 56.5 KB | Display | ![]() |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 1071 2: RESIDUES MET 1051, MET 1076 AND ARG 1131 HAVE TWO MODELED SIDE-CHAIN CONFORMATIONS. |
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Components
#1: Protein | Mass: 34792.805 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() | ||||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Nonpolymer details | THE MODEL INCLUDES TWO ETHYL MERCURY GROUPS (EMC) WHICH ARE COVALENTLY | Sequence details | RESIDUE NUMBERING IS ACCORDING TO EBINA ET AL. (REFERENCE 2). | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.3 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 21 ℃ / Method: vapor diffusion, hanging drop / Details: using macroseeding | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 37359 / % possible obs: 93.9 % |
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 20 Å / Observed criterion σ(I): 20.7 / Rmerge(I) obs: 0.027 |
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Processing
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Refinement | Resolution: 2.1→6 Å / σ(F): 2
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Displacement parameters | Biso mean: 23.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→6 Å
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