+Open data
-Basic information
Entry | Database: PDB / ID: 1igq | ||||||
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Title | C-terminal Domain of Transcriptional Repressor Protein KorB | ||||||
Components | Transcriptional repressor protein KorB | ||||||
Keywords | TRANSCRIPTION / SH3 domain / dimerization domain | ||||||
Function / homology | Function and homology information positive regulation of sporulation resulting in formation of a cellular spore / chromosome segregation / chromosome / negative regulation of DNA-templated transcription / protein-containing complex / DNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 1.7 Å | ||||||
Authors | Delbruck, H. / Heinemann, U. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4. Authors: Delbruck, H. / Ziegelin, G. / Lanka, E. / Heinemann, U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1igq.cif.gz | 61.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1igq.ent.gz | 46.7 KB | Display | PDB format |
PDBx/mmJSON format | 1igq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/1igq ftp://data.pdbj.org/pub/pdb/validation_reports/ig/1igq | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 7110.020 Da / Num. of mol.: 4 / Fragment: C-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: korb / Production host: Escherichia coli (E. coli) / References: UniProt: P07674 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.95 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: PEG4000, NaCl, Na acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.6 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X31 / Wavelength: 1.0721 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 25, 1997 / Details: crystal |
Radiation | Monochromator: Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0721 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→22 Å / Num. all: 32388 / Num. obs: 32160 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Biso Wilson estimate: 19.5 Å2 / Rmerge(I) obs: 0.044 / Net I/σ(I): 10.4 |
Reflection shell | Resolution: 1.7→1.79 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.203 / Mean I/σ(I) obs: 3.8 / Num. unique all: 4717 / % possible all: 95.7 |
Reflection | *PLUS Lowest resolution: 22 Å |
Reflection shell | *PLUS % possible obs: 95.7 % |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 1.7→22 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 22.62 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→22 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 22 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor Rfree: 0.228 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |