[English] 日本語
Yorodumi- PDB-1i7f: CRYSTAL STRUCTURE OF THE HSP33 DOMAIN WITH CONSTITUTIVE CHAPERONE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1i7f | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF THE HSP33 DOMAIN WITH CONSTITUTIVE CHAPERONE ACTIVITY | ||||||
Components | HEAT SHOCK PROTEIN 33 | ||||||
Keywords | CHAPERONE / Hsp33 / redox sensitive molecular chaperone | ||||||
Function / homology | Function and homology information maintenance of unfolded protein / protein folding chaperone / unfolded protein binding / response to heat / protein refolding / response to oxidative stress / zinc ion binding / identical protein binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.7 Å | ||||||
Authors | Kim, S.-J. / Jeong, D.-G. / Chi, S.-W. / Lee, J.-S. / Ryu, S.-E. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2001 Title: Crystal structure of proteolytic fragments of the redox-sensitive Hsp33 with constitutive chaperone activity Authors: Kim, S.J. / Jeong, D.G. / Chi, S.W. / Lee, J.S. / Ryu, S.E. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1i7f.cif.gz | 59.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1i7f.ent.gz | 42.7 KB | Display | PDB format |
PDBx/mmJSON format | 1i7f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1i7f_validation.pdf.gz | 451.8 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1i7f_full_validation.pdf.gz | 457.8 KB | Display | |
Data in XML | 1i7f_validation.xml.gz | 11.2 KB | Display | |
Data in CIF | 1i7f_validation.cif.gz | 14.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i7/1i7f ftp://data.pdbj.org/pub/pdb/validation_reports/i7/1i7f | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 32885.496 Da / Num. of mol.: 1 / Fragment: THE DOMAIN WITH CONSTITUTIVE CHAPERONE ACTIVITY / Mutation: C141D, C239S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: HSP33 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P0A6Y5 | ||||||
---|---|---|---|---|---|---|---|
#2: Chemical | #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | Has protein modification | Y | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.59 % | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 25 ℃ | |||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 93 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9879 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9879 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→99 Å / Num. all: 75273 / Num. obs: 10156 / % possible obs: 99.4 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 11.1 |
Reflection | *PLUS Num. measured all: 75273 |
Reflection shell | *PLUS % possible obs: 97.1 % / Rmerge(I) obs: 0.348 |
-Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MAD / Resolution: 2.7→99 Å / σ(F): 0 / σ(I): 0
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→99 Å
| ||||||||||||||||
Refine LS restraints |
| ||||||||||||||||
Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||
Refinement | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 99 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.222 / Rfactor Rfree: 0.29 | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS
|