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- PDB-1i2x: 2.4 A STRUCTURE OF A-DUPLEX WITH BULGED ADENOSINE, SPERMIDINE FORM -
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Open data
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Basic information
Entry | Database: PDB / ID: 1i2x | ||||||||||||||||||
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Title | 2.4 A STRUCTURE OF A-DUPLEX WITH BULGED ADENOSINE, SPERMIDINE FORM | ||||||||||||||||||
![]() | DNA/RNA (5'-R(*![]() DNA-RNA HYBRID / 'adenine bulge / A-type NA / DNA/RNA hybrid' | Function / homology | SPERMIDINE / DNA/RNA hybrid / DNA/RNA hybrid (> 10) | ![]() Method | ![]() ![]() ![]() Tereshko, V. / Wallace, S. / Usman, N. / Wincott, F. / Egli, M. | ![]() ![]() Title: X-ray crystallographic observation of "in-line" and "adjacent" conformations in a bulged self-cleaving RNA/DNA hybrid. Authors: Tereshko, V. / Wallace, S.T. / Usman, N. / Wincott, F.E. / Egli, M. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 35.6 KB | Display | ![]() |
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PDB format | ![]() | 24.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.2 KB | Display | ![]() |
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Full document | ![]() | 409.5 KB | Display | |
Data in XML | ![]() | 5.1 KB | Display | |
Data in CIF | ![]() | 6.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: DNA/RNA hybrid | Mass: 3471.194 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: Chemical | ChemComp-SPD / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.07 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6 Details: spermidine, MPD. pH 6.0, VAPOR DIFFUSION, SITTING DROP at 298 | |||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 9 / PH range high: 5.5 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Nov 26, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→20 Å / Num. all: 6220 / Num. obs: 6220 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection | *PLUS Num. obs: 6180 / % possible obs: 94.6 % / Rmerge(I) obs: 0.079 |
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Processing
Software |
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.4→10 Å
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 10 Å / σ(F): 2 / % reflection Rfree: 10 % / Rfactor obs: 0.249 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 2.49 Å / Rfactor Rfree: 0.358 / Rfactor Rwork: 0.332 / Num. reflection Rwork: 594 |