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Open data
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Basic information
Entry | Database: PDB / ID: 1hxd | ||||||
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Title | CRYSTAL STRUCTURE OF E. COLI BIOTIN REPRESSOR WITH BOUND BIOTIN | ||||||
![]() | BIRA BIFUNCTIONAL PROTEIN | ||||||
![]() | LIGASE / Repressor / BIOTIN / DNA-Binding | ||||||
Function / homology | ![]() biotin metabolic process / biotin-[biotin carboxyl-carrier protein] ligase / biotin--[biotin carboxyl-carrier protein] ligase activity / biotin biosynthetic process / biotin binding / transcription repressor complex / protein modification process / nucleic acid binding / transcription cis-regulatory region binding / regulation of DNA-templated transcription ...biotin metabolic process / biotin-[biotin carboxyl-carrier protein] ligase / biotin--[biotin carboxyl-carrier protein] ligase activity / biotin biosynthetic process / biotin binding / transcription repressor complex / protein modification process / nucleic acid binding / transcription cis-regulatory region binding / regulation of DNA-templated transcription / protein homodimerization activity / DNA binding / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kwon, K. / Streaker, E.D. / Ruparelia, S. / Beckett, D. | ||||||
![]() | ![]() Title: Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator. Authors: Weaver, L.H. / Kwon, K. / Beckett, D. / Matthews, B.W. #1: ![]() Title: MULTIPLE DISORDERED LOOPS FUNCTION IN COREPRESOR-INDUCED DIMERIZATION OF THE BIOTIN REPRESSOR Authors: Kwon, K. / Streaker, E.D. / Ruparelia, S. / Beckett, D. #2: ![]() Title: E. COLI BIOTIN HOLOENZYME SYNTHETASE/BIO REPRESSOR CRYSTAL STRUCTURE DELINEATES THE BIOTIN- AND DNA-BINDING DOMAINS Authors: Wilson, K.P. / Shewchuk, L.M. / Brennan, R.G. / Otsuka, A.J. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 131.3 KB | Display | ![]() |
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PDB format | ![]() | 102.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.4 KB | Display | ![]() |
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Full document | ![]() | 527.4 KB | Display | |
Data in XML | ![]() | 33.9 KB | Display | |
Data in CIF | ![]() | 45.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1biaS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35351.918 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P06709, biotin-[biotin carboxyl-carrier protein] ligase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.46 % | |||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() |
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Detector | Type: UCSD MARK II / Detector: AREA DETECTOR / Date: Sep 2, 1999 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→13 Å / Num. all: 48934 / Num. obs: 26762 / % possible obs: 83 % / Rmerge(I) obs: 0.071 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.4→2.59 Å / Rmerge(I) obs: 0.183 / Mean I/σ(I) obs: 1.8 / Num. unique all: 4098 |
Reflection | *PLUS % possible obs: 83 % / Num. measured all: 48934 |
Reflection shell | *PLUS % possible obs: 33 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1BIA Resolution: 2.4→13 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→13 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 13 Å / σ(F): 0 / Rfactor all: 0.189 | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS Type: t_angle_deg / Dev ideal: 2.8 |