integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / 2-LTR circle formation / Uncoating of the HIV Virion / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus ...integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / 2-LTR circle formation / Uncoating of the HIV Virion / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / Binding and entry of HIV virion / viral life cycle / symbiont-mediated activation of host apoptosis / HIV-1 retropepsin / Assembly Of The HIV Virion / retroviral ribonuclease H / exoribonuclease H / Budding and maturation of HIV virion / exoribonuclease H activity / protein processing / host multivesicular body / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / host cell / RNA-DNA hybrid ribonuclease activity / 転移酵素; リンを含む基を移すもの; 核酸を移すもの / symbiont-mediated suppression of host gene expression / peptidase activity / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / 加水分解酵素; エステル加水分解酵素 / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / DNA binding / zinc ion binding / identical protein binding / membrane 類似検索 - 分子機能
DETAILS OF THE CLONING AND EXPRESSION ARE PUBLISHED [M.C. GRAVES, J.J. LIM, E.P. HEIMER & R.A. ...DETAILS OF THE CLONING AND EXPRESSION ARE PUBLISHED [M.C. GRAVES, J.J. LIM, E.P. HEIMER & R.A. KRAMER (1988) PROC. NATL. ACAD. SCI., USA 85, 2449-2453]. PROTEIN WAS REFOLDED FROM THE INSOLUBLE FRACTION OF THE CELL LYSATE. THERE APPEARS TO BE NO SEQUENCE DATABASE REFERENCE TO THE HXB-3 VARIANT OF THE PROTEINASE SO A CROSS-REFERENCE IS GIVEN FOR THE HXB-2 VARIANT (IDENTICAL IN THE CODING REGION FOR THE PROTEINASE)
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.17 Å3/Da / 溶媒含有率: 43.4 % 解説: THE DATA REDUNDANCY IS 4.6 FOR DATA TO 2.01 ANGSTROMS RESOLUTION. R MERGE IS 0.035 FOR DATA TO 2.01 ANGSTROMS RESOLUTION. THE NUMBER OF UNIQUE REFLECTIONS IS 11337 TO 2.01 ANGSTROMS RESOLUTION.
結晶化
*PLUS
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データ収集
回折
平均測定温度: 298 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RUH2R / 波長: 1.5418
検出器
タイプ: ADSC / 検出器: AREA DETECTOR / 日付: 1990年9月15日
放射
モノクロメーター: GRAPHITE(002) / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
% possible obs: 91.6 % / Observed criterion σ(I): 0
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解析
ソフトウェア
名称
バージョン
分類
X-PLOR
2.1
モデル構築
X-PLOR
2.1
精密化
ADSC
データ収集
X-PLOR
2.1
位相決定
精密化
解像度: 2.3→10 Å / σ(F): 0 詳細: THE STEREOCHEMICAL PARAMETERS USED WERE THOSE DISTRIBUTED WITH VERSION 2.1 OF X-PLOR. THE ASYMMETRIC UNIT CONSISTS OF THE PROTEIN DIMER AND ONE INHIBITOR MOLECULE. THE INHIBITOR HAS BEEN ...詳細: THE STEREOCHEMICAL PARAMETERS USED WERE THOSE DISTRIBUTED WITH VERSION 2.1 OF X-PLOR. THE ASYMMETRIC UNIT CONSISTS OF THE PROTEIN DIMER AND ONE INHIBITOR MOLECULE. THE INHIBITOR HAS BEEN PLACED IN TWO ORIENTATIONS WITH OCCUPANCIES OF 0.56 AND 0.44 WHICH WERE CHOSEN TO MAKE THE TWO SETS OF TEMPERATURE FACTORS MORE EQUIVALENT. RESTRAINTS FROM NON-CRYSTALLOGRAPHIC SYMMETRY WERE NOT USED IN REFINEMENT.