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Open data
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Basic information
Entry | Database: PDB / ID: 1hw5 | ||||||
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Title | THE CAP/CRP VARIANT T127L/S128A | ||||||
![]() | CATABOLITE GENE ACTIVATOR | ||||||
![]() | GENE REGULATION / cAMP Receptor Protein / Catabolite Activator Protein (CAP) Transcription / Allostery / cAMP / cyclic AMP Mutant | ||||||
Function / homology | ![]() carbon catabolite repression of transcription / DNA binding, bending / minor groove of adenine-thymine-rich DNA binding / cAMP binding / protein-DNA complex / sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA-templated transcription / positive regulation of DNA-templated transcription ...carbon catabolite repression of transcription / DNA binding, bending / minor groove of adenine-thymine-rich DNA binding / cAMP binding / protein-DNA complex / sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA-templated transcription / positive regulation of DNA-templated transcription / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chu, S.Y. / Tordova, M. / Gilliland, G.L. / Gorshkova, I. / Shi, Y. | ||||||
![]() | ![]() Title: The structure of the T127L/S128A mutant of cAMP receptor protein facilitates promoter site binding Authors: Chu, S.Y. / Tordova, M. / Gilliland, G.L. / Gorshkova, I. / Shi, Y. / Wang, S. / Schwarz, F.P. #1: ![]() Title: The Structure of a CAP-DNA Complex Having Two cAMP Molecules Bound To Each Monomer Authors: Passner, J.M. / Steitz, T.A. #2: ![]() Title: Structure of a Complex of Catabolite Gene Activator Protein and Cyclic AMP Refined at 2.5 A Resolution Authors: Weber, I.T. / Steitz, T.A. #3: ![]() Title: Crystal Structure of a Cyclic AMP-independent Mutant of Catabolite Gene Activator Protein Authors: Weber, I.T. / Gilliland, G.L. / Harman, J.G. / Peterkofsky, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.5 KB | Display | ![]() |
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PDB format | ![]() | 78.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 22.7 KB | Display | |
Data in CIF | ![]() | 31.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3gap S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The dimer in the asymmetric unit is the biological unit |
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Components
#1: Protein | Mass: 23668.494 Da / Num. of mol.: 2 / Mutation: T127L, S128A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.01 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.1 Details: PEG 4K, isoprotanol, cAMP, HEPES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: Mar 12, 1998 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.82→20 Å / Num. all: 38054 / Num. obs: 177444 / % possible obs: 98 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 5 % / Biso Wilson estimate: 20 Å2 / Rmerge(I) obs: 0.088 / Net I/σ(I): 12 |
Reflection shell | Resolution: 1.82→20 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.088 / % possible all: 98 |
Reflection | *PLUS Num. obs: 38054 / % possible obs: 97.7 % / Redundancy: 4.7 % / Num. measured all: 177444 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3GAP ![]() 3gap Resolution: 1.82→20 Å / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.82→20 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 1 / Rfactor Rfree: 0.3 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS Type: s_plane_restr / Dev ideal: 0.025 |