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- PDB-1hmy: CRYSTAL STRUCTURE OF THE HHAI DNA METHYLTRANSFERASE COMPLEXED WIT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hmy | ||||||
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Title | CRYSTAL STRUCTURE OF THE HHAI DNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYL-L-METHIONINE | ||||||
![]() | HaeIII METHYLTRANSFERASE | ||||||
![]() | TRANSFERASE(METHYLTRANSFERASE) | ||||||
Function / homology | ![]() DNA (cytosine-5-)-methyltransferase / DNA (cytosine-5-)-methyltransferase activity / DNA restriction-modification system / methylation / DNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Cheng, X. | ||||||
![]() | ![]() Title: Crystal structure of the HhaI DNA methyltransferase complexed with S-adenosyl-L-methionine. Authors: Cheng, X. / Kumar, S. / Posfai, J. / Pflugrath, J.W. / Roberts, R.J. #1: ![]() Title: Purification, Crystallization, and Preliminary X-Ray Diffraction Analysis of an M.HhaI-Adomet Complex Authors: Kumar, S. / Cheng, X. / Pflugrath, J.W. / Roberts, R.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 76.4 KB | Display | ![]() |
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PDB format | ![]() | 57.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438 KB | Display | ![]() |
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Full document | ![]() | 448.2 KB | Display | |
Data in XML | ![]() | 10 KB | Display | |
Data in CIF | ![]() | 13.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 113 2: RESIDUES 85 - 91 ARE FLEXIBLE WITH HIGH TEMPERATURE FACTORS. |
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Components
#1: Protein | Mass: 37042.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() References: UniProt: P05102, DNA (cytosine-5-)-methyltransferase |
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#2: Chemical | ChemComp-SAM / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.82 Å3/Da / Density % sol: 74.48 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 16 ℃ / pH: 6.6 / Method: vapor diffusion, hanging drop / Details: referred to Biochemistry 31.8648-8653 1992 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 20981 / Num. measured all: 43292 / Rmerge(I) obs: 0.0719 |
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Processing
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Refinement | Rfactor Rwork: 0.2 / Rfactor obs: 0.2 / Highest resolution: 2.5 Å Details: RESIDUES 85 - 91 ARE FLEXIBLE WITH HIGH TEMPERATURE FACTORS. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 8 Å / Num. reflection obs: 20057 / σ(F): 2 / Rfactor obs: 0.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.82 |