PDB-1hls: NMR STRUCTURE OF THE HUMAN INSULIN-HIS(B16)

基本情報

• 登録情報: データベース: PDB / ID: 1hls
• タイトル: NMR STRUCTURE OF THE HUMAN INSULIN-HIS(B16)
• 要素: (INSULIN) x 2
• キーワード: HORMONE

機能・相同性

分子機能:

negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / Signaling by Insulin receptor / negative regulation of feeding behavior / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / positive regulation of protein autophosphorylation / alpha-beta T cell activation / positive regulation of dendritic spine maintenance / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / negative regulation of gluconeogenesis / positive regulation of glycogen biosynthetic process / Signal attenuation / fatty acid homeostasis / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / positive regulation of insulin receptor signaling pathway / negative regulation of lipid catabolic process / regulation of protein localization to plasma membrane / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / activation of protein kinase B activity / COPI-mediated anterograde transport / transport vesicle / nitric oxide-cGMP-mediated signaling / negative regulation of reactive oxygen species biosynthetic process / Insulin receptor recycling / insulin-like growth factor receptor binding / positive regulation of brown fat cell differentiation / NPAS4 regulates expression of target genes / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of nitric-oxide synthase activity / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / regulation of transmembrane transporter activity / positive regulation of glycolytic process / positive regulation of long-term synaptic potentiation / positive regulation of cytokine production / endosome lumen / acute-phase response / positive regulation of D-glucose import / positive regulation of protein secretion / positive regulation of cell differentiation / Regulation of insulin secretion / insulin receptor binding / wound healing / negative regulation of protein catabolic process / hormone activity / regulation of synaptic plasticity / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / cognition / glucose metabolic process / vasodilation / insulin receptor signaling pathway / glucose homeostasis / cell-cell signaling / regulation of protein localization / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protease binding / positive regulation of cell growth / secretory granule lumen / positive regulation of canonical NF-kappaB signal transduction / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of MAPK cascade / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding

ドメイン・相同性:

Insulin / Insulin family / Insulin-like / Insulin/IGF/Relaxin family / Insulin / insulin-like growth factor / relaxin family. / Insulin, conserved site / Insulin family signature. / Insulin-like superfamily

構成要素:

Insulin

• 生物種: Homo sapiens (ヒト)
• 手法: 溶液NMR
• データ登録者: Ludvigsen, S. / Kaarsholm, N.C.
• 引用: ジャーナル: Biochemistry / 年: 1994; タイトル: High-resolution structure of an engineered biologically potent insulin monomer, B16 Tyr-->His, as determined by nuclear magnetic resonance spectroscopy.; 著者: Ludvigsen, S. / Roy, M. / Thogersen, H. / Kaarsholm, N.C.

履歴

登録	1995年6月28日	処理サイト: BNL
改定 1.0	1995年9月15日	Provider: repository / タイプ: Initial release
改定 1.1	2008年3月24日	Group: Version format compliance
改定 1.2	2011年7月13日	Group: Version format compliance
改定 1.3	2017年11月29日	Group: Derived calculations / Other カテゴリ: pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf / struct_conf_type Item: _pdbx_database_status.process_site
改定 1.4	2024年10月23日	Group: Data collection / Database references / Structure summary カテゴリ: chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / struct_ref_seq_dif Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

集合体

登録構造単位

A: INSULIN

B: INSULIN

概要:

• 5.79 kDa, 2 ポリマー

構成要素の詳細:

	分子量 (理論値)	分子数
合計 (水以外)	5,793	2
ポリマ－	5,793	2
非ポリマー	0	0
水	0	0

1

概要:

• 登録構造と同一
• 登録者が定義した集合体

対称操作:

タイプ	名称	対称操作	数
identity operation	1_555	x,y,z	1

NMR アンサンブル

	データ	基準
コンフォーマー数 (登録 / 計算)	20 / -
代表モデル

要素

#1: タンパク質・ペプチド

A INSULIN

詳細:

分子量: 2383.698 Da / 分子数: 1 / 変異: TYR 16 B HIS / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 器官: PANCREAS / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308

#2: タンパク質・ペプチド

B INSULIN

詳細:

分子量: 3408.926 Da / 分子数: 1 / 変異: TYR 16 B HIS / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 器官: PANCREAS / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308

• Has protein modification: Y

実験情報

実験

• 実験: 手法: 溶液NMR

試料調製

• 結晶化: 手法: other / 詳細: NMR

解析

ソフトウェア

名称	バージョン	分類
X-PLOR	3	モデル構築
X-PLOR	3	精密化
X-PLOR	3	位相決定

• NMR software: 名称: X-PLOR / バージョン: 3 / 開発者: BRUNGER / 分類: 精密化
• NMRアンサンブル: 登録したコンフォーマーの数: 20