+Open data
-Basic information
Entry | Database: PDB / ID: 1hky | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of a PAN module from Eimeria tenella | ||||||
Components | MICRONEME PROTEIN 5 PRECURSOR | ||||||
Keywords | ADHESIN / PAN MODULE / EIMERIA TENELLA | ||||||
Function / homology | Function and homology information | ||||||
Biological species | EIMERIA TENELLA (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Brown, P.J. / Mulvey, D. / Potts, J.R. / Tomley, F.M. / Campbell, I.D. | ||||||
Citation | Journal: J.Struct.Funct.Genom. / Year: 2003 Title: Solution Structure of a Pan Module from the Apicomplexan Parasite Eimeria Tenella Authors: Brown, P.J. / Mulvey, D. / Potts, J.R. / Tomley, F.M. / Campbell, I.D. | ||||||
History |
| ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1hky.cif.gz | 488 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1hky.ent.gz | 416.9 KB | Display | PDB format |
PDBx/mmJSON format | 1hky.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hky_validation.pdf.gz | 345.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1hky_full_validation.pdf.gz | 492 KB | Display | |
Data in XML | 1hky_validation.xml.gz | 27.2 KB | Display | |
Data in CIF | 1hky_validation.cif.gz | 44.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hk/1hky ftp://data.pdbj.org/pub/pdb/validation_reports/hk/1hky | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 9233.349 Da / Num. of mol.: 1 / Fragment: RESIDUES 712-786 Source method: isolated from a genetically manipulated source Details: CONSTRUCT INCLUDES 11 NON-NATIVE RESIDUES N-TERMINAL TO THR712 Source: (gene. exp.) EIMERIA TENELLA (eukaryote) / Strain: HOUGHTON Description: ETMIC5 IS A SECRETED PROTEIN FROM THE MICRONEME ORGANELLES Plasmid: PFLAG-ATS / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q9U966 |
---|---|
Sequence details | 11 NON-NATIVE RESIDUES N-TERMINAL TO THR712 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||
NMR details | Text: STRUCTURE WAS DETERMINED USING HETERONUCLEAR NMR SPECTROSCOPY ON 15N-LABELLED 9TH PAN MODULE FROM ETMIC5. MODEL 1 HAS LOWEST ENERGY |
-Sample preparation
Details | Contents: 0.8 MM IN 93% H2O/7% D2O OR 1.7 MM IN 99.9% D2O |
---|---|
Sample conditions | pH: 4.7 / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 / Details: REFINEMENT DETAILS WILL BE PUBLISHED | |||||||||
NMR ensemble | Conformer selection criteria: LOW ENERGY AND LEAST RESTRAINT VIOLATIONS Conformers calculated total number: 250 / Conformers submitted total number: 20 |