Mass: 9233.349 Da / Num. of mol.: 1 / Fragment: RESIDUES 712-786 Source method: isolated from a genetically manipulated source Details: CONSTRUCT INCLUDES 11 NON-NATIVE RESIDUES N-TERMINAL TO THR712 Source: (gene. exp.) EIMERIA TENELLA (eukaryote) / Strain: HOUGHTON Description: ETMIC5 IS A SECRETED PROTEIN FROM THE MICRONEME ORGANELLES Plasmid: PFLAG-ATS / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q9U966
Sequence details
11 NON-NATIVE RESIDUES N-TERMINAL TO THR712
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
TOCSY
1
3
1
COSY
1
4
1
1H-15N-NOESY-HSQC 1H-15N-TOCSY-HSQC
1
5
1
HMQCJ
NMR details
Text: STRUCTURE WAS DETERMINED USING HETERONUCLEAR NMR SPECTROSCOPY ON 15N-LABELLED 9TH PAN MODULE FROM ETMIC5. MODEL 1 HAS LOWEST ENERGY
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Sample preparation
Details
Contents: 0.8 MM IN 93% H2O/7% D2O OR 1.7 MM IN 99.9% D2O
Sample conditions
pH: 4.7 / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Field strength (MHz)
Spectrometer-ID
500
1
600
2
750
3
-
Processing
NMR software
Name
Developer
Classification
X-PLOR3.8
BRUNGER
refinement
XPLOR3.8
structuresolution
Refinement
Method: simulated annealing / Software ordinal: 1 / Details: REFINEMENT DETAILS WILL BE PUBLISHED
NMR ensemble
Conformer selection criteria: LOW ENERGY AND LEAST RESTRAINT VIOLATIONS Conformers calculated total number: 250 / Conformers submitted total number: 20
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