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- PDB-1hj1: RAT OESTROGEN RECEPTOR BETA LIGAND-BINDING DOMAIN IN COMPLEX WITH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hj1 | ||||||
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Title | RAT OESTROGEN RECEPTOR BETA LIGAND-BINDING DOMAIN IN COMPLEX WITH PURE ANTIOESTROGEN ICI164,384 | ||||||
![]() | OESTROGEN RECEPTOR BETA | ||||||
![]() | NUCLEAR RECEPTOR / TRANSCRIPTION FACTOR / OESTROGEN / ANTAGONIST | ||||||
Function / homology | ![]() negative regulation of behavior / cellular response to magnetism / response to bisphenol A / ESR-mediated signaling / PIP3 activates AKT signaling / estrogen binding / estradiol binding / response to insecticide / epithelial cell maturation involved in prostate gland development / amygdala development ...negative regulation of behavior / cellular response to magnetism / response to bisphenol A / ESR-mediated signaling / PIP3 activates AKT signaling / estrogen binding / estradiol binding / response to insecticide / epithelial cell maturation involved in prostate gland development / amygdala development / response to human chorionic gonadotropin / hormone-mediated apoptotic signaling pathway / Extra-nuclear estrogen signaling / Sertoli cell proliferation / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Sertoli cell development / prostate gland development / organic cyclic compound binding / response to genistein / Nuclear Receptor transcription pathway / negative regulation of androgen receptor signaling pathway / steroid hormone binding / hormone binding / response to salt / prostate gland epithelium morphogenesis / peroxisome proliferator activated receptor binding / positive regulation of epidermal growth factor receptor signaling pathway / nuclear steroid receptor activity / hypothalamus development / negative regulation of feeding behavior / heterocyclic compound binding / female gonad development / response to testosterone / nuclear estrogen receptor activity / response to dexamethasone / androgen receptor signaling pathway / uterus development / vagina development / cellular response to organic cyclic compound / estrous cycle / negative regulation of reactive oxygen species metabolic process / behavioral fear response / estrogen receptor signaling pathway / cellular response to estrogen stimulus / estrogen response element binding / nuclear receptor-mediated steroid hormone signaling pathway / ovarian follicle development / steroid binding / response to nutrient levels / cerebellum development / response to hormone / epithelial cell proliferation / response to activity / cellular response to estradiol stimulus / promoter-specific chromatin binding / protein-DNA complex / negative regulation of smooth muscle cell proliferation / neuron migration / response to organic cyclic compound / brain development / positive regulation of DNA-binding transcription factor activity / response to estrogen / vasodilation / nuclear receptor activity / negative regulation of epithelial cell proliferation / male gonad development / cellular response to xenobiotic stimulus / response to estradiol / regulation of cell population proliferation / DNA-binding transcription activator activity, RNA polymerase II-specific / perikaryon / cellular response to lipopolysaccharide / response to ethanol / cell population proliferation / negative regulation of neuron apoptotic process / sequence-specific DNA binding / learning or memory / positive regulation of ERK1 and ERK2 cascade / response to xenobiotic stimulus / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of cell population proliferation / neuronal cell body / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / perinuclear region of cytoplasm / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / zinc ion binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pike, A.C.W. / Brzozowski, A.M. / Carlquist, M. | ||||||
![]() | ![]() Title: Structural Insights Into the Mode of Action of a Pure Antiestrogen Authors: Pike, A.C.W. / Brzozowski, A.M. / Walton, J. / Hubbard, R.E. / Thorsell, A.G. / Li, Y.L. / Gustafsson, J.A. / Carlquist, M. #1: Journal: Biochem.Soc.Trans. / Year: 2001 Title: Structural Aspects of Agonism and Antagonism in the Oestrogen Receptor Authors: Pike, A.C. / Brzozowski, A.M. / Walton, J. / Hubbard, R.E. / Bonn, T. / Gustafsson, J.A. / Carlquist, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.1 KB | Display | ![]() |
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PDB format | ![]() | 56.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 997.3 KB | Display | ![]() |
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Full document | ![]() | 995.6 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 14.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1qknS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | BIOMOLECULEHOMODIMER |
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Components
#1: Protein | Mass: 28686.002 Da / Num. of mol.: 1 / Fragment: LIGAND-BINDING DOMAIN Source method: isolated from a genetically manipulated source Details: COMPLEXED WITH THE FULL ANTAGONIST ICI164,384 / Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-AOE / | ||
#3: Chemical | ChemComp-PMB / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: 7.0% (W/V) PEG 2000 MONOMETHYL ETHER 0.0035M NICKEL CHLORIDE 0.035M TRIS-HCL, PH 8.5 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 23, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9464 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. obs: 12201 / % possible obs: 98.2 % / Observed criterion σ(I): -3 / Redundancy: 4 % / Biso Wilson estimate: 48.5 Å2 / Rsym value: 0.06 / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 2.3→2.34 Å / Redundancy: 4 % / Mean I/σ(I) obs: 2 / Rsym value: 0.326 / % possible all: 98.6 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. measured all: 185069 / Rmerge(I) obs: 0.06 |
Reflection shell | *PLUS % possible obs: 98.6 % / Rmerge(I) obs: 0.326 / Mean I/σ(I) obs: 2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1QKN Resolution: 2.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.31611 / ESU R Free: 0.22821 Details: BULK SOLVENT CORRECTION CALCULATED IN XPLOR V3.843 WAS USED THROUGHOUT REFINEMENT. TEMPERATURE FACTORS OF HG OF RESIDUE PMB A1437 REFINED ANISOTROPICALLY. ISOTROPIC MODEL USED FOR REMAINING ...Details: BULK SOLVENT CORRECTION CALCULATED IN XPLOR V3.843 WAS USED THROUGHOUT REFINEMENT. TEMPERATURE FACTORS OF HG OF RESIDUE PMB A1437 REFINED ANISOTROPICALLY. ISOTROPIC MODEL USED FOR REMAINING ATOMS. RESIDUES AFTER LYS435 ARE NOT VISIBLE IN THE ELECTRON DENSITY MAPS. PCMBS MOLECULE COVALENTLY BOUND (PMB) TO CYS289
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Displacement parameters | Biso mean: 53 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Rfactor obs: 0.21966 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |