[English] 日本語
![](img/lk-miru.gif)
- PDB-1hi7: NMR SOLUTION STRUCTURE OF THE DISULPHIDE-LINKED HOMODIMER OF HUMA... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1hi7 | ||||||
---|---|---|---|---|---|---|---|
Title | NMR SOLUTION STRUCTURE OF THE DISULPHIDE-LINKED HOMODIMER OF HUMAN TFF1, 10 STRUCTURES | ||||||
![]() | PS2 PROTEIN | ||||||
![]() | GROWTH FACTOR / CELL MOTILITY / TUMOR SUPPRESSOR / TREFOIL DOMAIN | ||||||
Function / homology | ![]() maintenance of gastrointestinal epithelium / response to iron ion / response to immobilization stress / growth factor activity / response to peptide hormone / Estrogen-dependent gene expression / cell population proliferation / carbohydrate metabolic process / cell differentiation / negative regulation of cell population proliferation ...maintenance of gastrointestinal epithelium / response to iron ion / response to immobilization stress / growth factor activity / response to peptide hormone / Estrogen-dependent gene expression / cell population proliferation / carbohydrate metabolic process / cell differentiation / negative regulation of cell population proliferation / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Williams, M.A. / Feeney, J. | ||||||
![]() | ![]() Title: The Solution Structure of the Disulphide-Linked Dimeric of the Human Trefoil Protein Tff1 Authors: Williams, M.A. / Westley, B.R. / May, F.E. / Feeney, J. #1: ![]() Title: High-Resolution Solution Structure of Human Pnr-2/ Ps2: A Single Trefoil Motif Protein Authors: Polshakov, V.I. / Williams, M.A. / Gargaro, A.R. / Frenkiel, T.A. / Westley, B.R. / Chadwick, M.P. / May, F.E.B. / Feeney, J. #2: Journal: Biochem.J. / Year: 1997 Title: Homodimerization and Hetero_Oligomerization of the Single Domain Trefoil Protein Pnr-2/Ps2 Through Cysteine 58 Authors: Chadwick, M.P. / May, F.E.B. / Westley, B.R. #3: Journal: Biochem.J. / Year: 1995 Title: Production and Comparison of Mature Single-Domain 'Trefoil' Peptides Pnr-2/Ps2 Cys58 and Pnr-2/Ps2 Ser58 Authors: Chadwick, M.P. / May, F.E.B. / Westley, B.R. #4: Journal: Eur.J.Biochem. / Year: 1995 Title: NMR-Based Structural Studies of the Pnr-2/Ps2 Single Domain Trefoil Peptide. Similarities to Porcine Spasmolytic Peptide and Evidence for a Monomeric Structure Authors: Polshakov, V.I. / Frenkiel, T.A. / Westley, B. / Chadwick, M. / May, F. / Carr, M.D. / Feeney, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 344.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 298.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 354.3 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 534.5 KB | Display | |
Data in XML | ![]() | 48.8 KB | Display | |
Data in CIF | ![]() | 64.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 6678.365 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: DISULPHIDE LINK BETWEEN C58 OF BOTH SUBUNITS / Source: (gene. exp.) ![]() ![]() ![]() Sequence details | 1PS2 SWS P04155 1 - 24 NOT IN ATOMS LIST | |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||
NMR details | Text: THE STRUCTURE WAS DETERMINED USING TWO AND THREE DIMENSIONAL NMR EXPERIMENTS ON, RESPECTIVELY, UNLABELLED AND N-15 LABELLED TFF1 DIMER PROTEIN. MANY ASSIGN WERE MADE BY MAKING USE IF THE BETTER ...Text: THE STRUCTURE WAS DETERMINED USING TWO AND THREE DIMENSIONAL NMR EXPERIMENTS ON, RESPECTIVELY, UNLABELLED AND N-15 LABELLED TFF1 DIMER PROTEIN. MANY ASSIGN WERE MADE BY MAKING USE IF THE BETTER RESOLVED MONOMER SPECTRA (POLSHAKOV ET AL. 1995,1997) |
-
Sample preparation
Sample conditions | Ionic strength: 0.01 / pH: 5.9 / Pressure: 1 atm / Temperature: 298 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-
Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: TOTAL NOE CONSTRAINT VIOLATIONS < 1.0 A Conformers calculated total number: 11 / Conformers submitted total number: 10 |