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- PDB-1h7f: The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h7f | ||||||
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Title | The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase and of its complexes with substrates and substrate analogues, CMP complex | ||||||
![]() | 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE | ||||||
![]() | NUCLEOTIDYLTRANSFERASE / CMP-KDO SYNTHETASE / NUCLEOSIDE MONOPHOSPHATE GLYCOSIDES / LIPOPOLYSACCHARIDE BIOSYNTHESIS / SUGAR-ACTIVATING ENZYMES | ||||||
Function / homology | ![]() 3-deoxy-manno-octulosonate cytidylyltransferase / 3-deoxy-manno-octulosonate cytidylyltransferase activity / CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process / lipopolysaccharide biosynthetic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Jelakovic, S. / Schulz, G.E. | ||||||
![]() | ![]() Title: The Structure of Cmp:2-Keto-3-Deoxy-Manno-Octonic Acid Synthetase and of its Complexes with Substrates and Substrate Analogs Authors: Jelakovic, S. / Schulz, G.E. #1: ![]() Title: The Three-Dimensional Structure of Capsule-Specific Cmp:2-Keto-3-Deoxy-Manno-Octonic Acid Synthetase from Escherichia Coli Authors: Jelakovic, S. / Jann, K. / Schulz, G.E. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 111.3 KB | Display | ![]() |
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PDB format | ![]() | 86.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 809.3 KB | Display | ![]() |
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Full document | ![]() | 814.1 KB | Display | |
Data in XML | ![]() | 23 KB | Display | |
Data in CIF | ![]() | 33.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.050529, 0.855681, -0.515031), Vector: |
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Components
#1: Protein | Mass: 27059.912 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P42216, 3-deoxy-manno-octulosonate cytidylyltransferase #2: Chemical | ChemComp-C5P / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.11 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 9.4 / Details: pH 9.40 | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 297 K |
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Diffraction source | Source: ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.12→24.7 Å / Num. obs: 30952 / % possible obs: 96 % / Redundancy: 2.6 % / Rsym value: 0.066 |
Reflection | *PLUS % possible obs: 96 % / Rmerge(I) obs: 0.066 |
Reflection shell | *PLUS Highest resolution: 2.12 Å / Lowest resolution: 2.19 Å / % possible obs: 89 % / Redundancy: 1.8 % / Num. unique obs: 2777 / Rmerge(I) obs: 0.15 / Mean I/σ(I) obs: 4.6 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 2.12→22 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.12→22 Å
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Refine LS restraints |
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