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Open data
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Basic information
Entry | Database: PDB / ID: 1guo | ||||||
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Title | MopII from Clostridium pasteurianum complexed with molybdate | ||||||
![]() | MOLYBDATE BINDING PROTEIN II | ||||||
![]() | TRANSPORT PROTEIN / MOLYBDATE BINDING / MOP / MOLYBDENUM | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Schuettelkopf, A.W. / Harrison, J.A. / Hunter, W.N. | ||||||
![]() | ![]() Title: Passive Acquisition of Ligand by the Mopii Molbindin from Clostridium Pasteurianum: Structures of Apo and Oxyanion-Bound Forms Authors: Schuettelkopf, A.W. / Harrison, J.A. / Boxer, D.H. / Hunter, W.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.6 KB | Display | ![]() |
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PDB format | ![]() | 64.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 475.4 KB | Display | ![]() |
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Full document | ![]() | 480.1 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 21.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gugSC ![]() 1gunC ![]() 1gusC ![]() 1gutC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 7017.250 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-MOO / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.6 Details: 95 MM HEPES PH 7.5, 27% POLYETHYLENE GLYCOL 400,5% GLYCEROL, 190 MM CACL2 WITH 1.6 MM NA2MOO4 IN THE DROP | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / pH: 7.5 / Method: vapor diffusion, hanging dropDetails: Harrison, J.A., (2001) Acta Crystallogr., D57, 1715. | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU IMAGE PLATE / Detector: IMAGE PLATE / Date: Jul 15, 2001 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 10097 / % possible obs: 98.3 % / Redundancy: 4 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 27.7 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.259 / Mean I/σ(I) obs: 4.4 / % possible all: 89.2 |
Reflection | *PLUS Num. measured all: 173881 / Rmerge(I) obs: 0.05 |
Reflection shell | *PLUS % possible obs: 89.2 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GUG Resolution: 2.5→29.88 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.92 / SU B: 13.65 / SU ML: 0.31 / Cross valid method: THROUGHOUT / ESU R Free: 0.356 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: THE DATA SET WAS ORIGINALLY PROCESSED/ SCALED IN AN ORTHORHOMBIC SPACE GROUP, BUT COULD NOT BE REFINED WITH THE ADDITIONAL CRYSTALLOGRAPHIC SYMMETRY.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→29.88 Å
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Refine LS restraints |
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