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Open data
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Basic information
Entry | Database: PDB / ID: 1guf | ||||||
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Title | Enoyl thioester reductase from Candida tropicalis | ||||||
![]() | ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADPH, B-SPECIFIC] 1, MITOCHONDRIAL | ||||||
![]() | OXIDOREDUCTASE | ||||||
Function / homology | ![]() enoyl-[acyl-carrier-protein] reductase (NADPH) / trans-2-enoyl-CoA reductase (NADPH) activity / fatty acid metabolic process / fatty acid biosynthetic process / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Airenne, T.T. / Torkko, J.M. / Van Der Plas, S. / Sormunen, R.T. / Kastaniotis, A.J. / Wierenga, R.K. / Hiltunen, J.K. | ||||||
![]() | ![]() Title: Structure-Function Analysis of Enoyl Thioester Reductase Involved in Mitochondrial Maintenance Authors: Airenne, T.T. / Torkko, J.M. / Van Der Plas, S. / Sormunen, R.T. / Kastaniotis, A.J. / Wierenga, R.K. / Hiltunen, J.K. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 167.3 KB | Display | ![]() |
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PDB format | ![]() | 132.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 36.1 KB | Display | |
Data in CIF | ![]() | 52 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gu7SC ![]() 1gyrC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.721, 0.6687, -0.1816), Vector: |
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Components
#1: Protein | Mass: 39555.402 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q8WZM3, EC: 1.3.1.10, trans-2-enoyl-CoA reductase (NADPH) #2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-SO4 / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.5 Å3/Da / Density % sol: 64.83 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.00 | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→25 Å / Num. obs: 53552 / % possible obs: 99.7 % / Redundancy: 6.9 % / Biso Wilson estimate: 18.8 Å2 / Rmerge(I) obs: 0.013 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 2.25→2.33 Å / Rmerge(I) obs: 0.04 / Mean I/σ(I) obs: 4.8 / % possible all: 99.2 |
Reflection | *PLUS Lowest resolution: 25 Å / Rmerge(I) obs: 0.013 |
Reflection shell | *PLUS % possible obs: 99.2 % / Num. unique obs: 5181 / Rmerge(I) obs: 0.402 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GU7 Resolution: 2.25→24.07 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 212740.53 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 34.3333 Å2 / ksol: 0.36995 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.25→24.07 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.33 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 25 Å / Rfactor Rfree: 0.216 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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