+Open data
-Basic information
Entry | Database: PDB / ID: 1gtu | ||||||
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Title | LIGAND-FREE HUMAN GLUTATHIONE S-TRANSFERASE M1A-1A | ||||||
Components | GLUTATHIONE S-TRANSFERASE | ||||||
Keywords | TRANSFERASE / GLUTATHIONE / CONJUGATION / DETOXIFICATION / CYTOSOLIC / DIMER | ||||||
Function / homology | Function and homology information nitrobenzene metabolic process / cellular detoxification of nitrogen compound / glutathione derivative biosynthetic process / glutathione binding / hepoxilin biosynthetic process / Glutathione conjugation / Paracetamol ADME / Azathioprine ADME / prostaglandin metabolic process / intercellular bridge ...nitrobenzene metabolic process / cellular detoxification of nitrogen compound / glutathione derivative biosynthetic process / glutathione binding / hepoxilin biosynthetic process / Glutathione conjugation / Paracetamol ADME / Azathioprine ADME / prostaglandin metabolic process / intercellular bridge / glutathione transferase / glutathione transferase activity / xenobiotic catabolic process / glutathione metabolic process / enzyme binding / protein homodimerization activity / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.68 Å | ||||||
Authors | Patskovsky, Y.V. / Patskovska, L.N. / Listowsky, I. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: Functions of His107 in the catalytic mechanism of human glutathione S-transferase hGSTM1a-1a. Authors: Patskovsky, Y.V. / Patskovska, L.N. / Listowsky, I. #1: Journal: Biochem.J. / Year: 1993 Title: Deduced Amino Acid Sequence, Gene Structure and Chromosomal Location of a Novel Human Class Mu Glutathione S-Transferase, Gstm4 Authors: Zhong, S. / Spurr, N.K. / Hayes, J.D. / Wolf, C.R. #2: Journal: Proc.Natl.Acad.Sci.USA / Year: 1988 Title: Hereditary Differences in the Expression of the Human Glutathione Transferase Active on Trans-Stilbene Oxide are due to a Gene Deletion Authors: Seidegard, J. / Vorachek, W.R. / Pero, R.W. / Pearson, W.R. #3: Journal: Nucleic Acids Res. / Year: 1988 Title: The Human Liver Glutathione S-Transferase Gene Superfamily: Expression and Chromosome Mapping of an Hb Subunit Cdna Authors: Dejong, J.L. / Chang, C.M. / Whang-Peng, J. / Knutsen, T. / TU, C.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gtu.cif.gz | 182.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gtu.ent.gz | 149.3 KB | Display | PDB format |
PDBx/mmJSON format | 1gtu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1gtu_validation.pdf.gz | 435.8 KB | Display | wwPDB validaton report |
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Full document | 1gtu_full_validation.pdf.gz | 443.4 KB | Display | |
Data in XML | 1gtu_validation.xml.gz | 30.9 KB | Display | |
Data in CIF | 1gtu_validation.cif.gz | 42.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gt/1gtu ftp://data.pdbj.org/pub/pdb/validation_reports/gt/1gtu | HTTPS FTP |
-Related structure data
Related structure data | 2gtuS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 25615.646 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: LIGAND-FREE / Source: (gene. exp.) Homo sapiens (human) Description: THE GSTM1A CDNA WAS AMPLIFIED USING RT-PCR AND SUBCLONED INTO A PET3A EXPRESSION VECTOR Cell line: HELA / Cellular location: CYTOPLASM / Gene: GSTM1A / Organ: LIVER / Plasmid: PET3A-GSTM1A / Species (production host): Escherichia coli / Gene (production host): GSTM1A / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P09488, glutathione transferase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 58 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 289 K / pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: SIEMENS-NICOLET X100 / Detector: AREA DETECTOR / Date: Apr 1, 1997 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.68→12.35 Å / Num. obs: 26217 / % possible obs: 98 % / Observed criterion σ(I): 2 / Redundancy: 2 % / Rmerge(I) obs: 0.058 |
Reflection shell | Resolution: 2.68→2.9 Å / Redundancy: 1.75 % / Rmerge(I) obs: 0.155 / % possible all: 87.1 |
Reflection shell | *PLUS % possible obs: 87.1 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2GTU Resolution: 2.68→10 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.68→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.68→2.8 Å / Rfactor Rfree error: 0.01 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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