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Open data
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Basic information
Entry | Database: PDB / ID: 1gsn | ||||||
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Title | HUMAN GLUTATHIONE REDUCTASE MODIFIED BY DINITROSOGLUTATHIONE | ||||||
![]() | GLUTATHIONE REDUCTASE | ||||||
![]() | OXIDOREDUCTASE / SULFHYDRYL OXIDATION / SULFENIC ACID / NITRIC OXIDE | ||||||
Function / homology | ![]() glutathione-disulfide reductase / Metabolism of ingested H2SeO4 and H2SeO3 into H2Se / glutathione-disulfide reductase (NADPH) activity / Interconversion of nucleotide di- and triphosphates / NFE2L2 regulating anti-oxidant/detoxification enzymes / Detoxification of Reactive Oxygen Species / glutathione metabolic process / cell redox homeostasis / TP53 Regulates Metabolic Genes / NADP binding ...glutathione-disulfide reductase / Metabolism of ingested H2SeO4 and H2SeO3 into H2Se / glutathione-disulfide reductase (NADPH) activity / Interconversion of nucleotide di- and triphosphates / NFE2L2 regulating anti-oxidant/detoxification enzymes / Detoxification of Reactive Oxygen Species / glutathione metabolic process / cell redox homeostasis / TP53 Regulates Metabolic Genes / NADP binding / flavin adenine dinucleotide binding / cellular response to oxidative stress / electron transfer activity / mitochondrial matrix / external side of plasma membrane / mitochondrion / extracellular exosome / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Becker, K. / Savvides, S.N. / Keese, M. / Schirmer, R.H. / Karplus, P.A. | ||||||
![]() | ![]() Title: Enzyme inactivation through sulfhydryl oxidation by physiologic NO-carriers. Authors: Becker, K. / Savvides, S.N. / Keese, M. / Schirmer, R.H. / Karplus, P.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 112.1 KB | Display | ![]() |
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PDB format | ![]() | 90.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1007.9 KB | Display | ![]() |
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Full document | ![]() | 1010.6 KB | Display | |
Data in XML | ![]() | 25.5 KB | Display | |
Data in CIF | ![]() | 38.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51700.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: MIXED DISULFIDE BETWEEN C58 AND GLUTATHIONE (GSH 1030) SULFENIC ACID GROUP IN CEA63, CEA234, CEA284, CEA423 Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-PO4 / |
#3: Chemical | ChemComp-FAD / |
#4: Chemical | ChemComp-GSH / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.59 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: May 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.7 Å / Num. obs: 57567 / % possible obs: 97 % / Redundancy: 4.1 % / Rsym value: 0.07 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 1.7→1.74 Å / Mean I/σ(I) obs: 2.5 / Rsym value: 0.328 / % possible all: 79 |
Reflection | *PLUS Num. measured all: 238736 / Rmerge(I) obs: 0.07 |
Reflection shell | *PLUS % possible obs: 79 % / Rmerge(I) obs: 0.328 |
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Processing
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Refinement | Resolution: 1.7→10 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 1.7→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |