- PDB-1gml: crystal structure of the mouse CCT gamma apical domain (triclinic) -
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データを開く
IDまたはキーワード:
読み込み中...
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基本情報
登録情報
データベース: PDB / ID: 1gml
タイトル
crystal structure of the mouse CCT gamma apical domain (triclinic)
要素
T-COMPLEX PROTEIN 1 SUBUNIT GAMMA
キーワード
CHAPERONE / CHAPERONIN / ACTIN / TUBULIN
機能・相同性
機能・相同性情報
Association of TriC/CCT with target proteins during biosynthesis / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / binding of sperm to zona pellucida / chaperonin-containing T-complex / chaperone-mediated protein folding / positive regulation of telomere maintenance via telomerase / ATP-dependent protein folding chaperone / unfolded protein binding ...Association of TriC/CCT with target proteins during biosynthesis / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / binding of sperm to zona pellucida / chaperonin-containing T-complex / chaperone-mediated protein folding / positive regulation of telomere maintenance via telomerase / ATP-dependent protein folding chaperone / unfolded protein binding / protein folding / myelin sheath / cell body / microtubule / protein stabilization / ATP hydrolysis activity / ATP binding 類似検索 - 分子機能
THE KNOWN BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATEOF THE MOLECULE IS THE MONOMER. TOGETHER WITH THE SEVEN OTHER SUBUNITS OF CCT, IT IS PART OF A DOUBLE TOROIDAL QUATERNARY STRUCTURE OF 2X8 SUBUNITS.
手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: CRYSTALS GROWN BY HANGING DROP METHOD USING A 1:1 MIXTURE OF 12MG/ML PROTEIN, 400MM NACL, 20% GLYCEROL, 8MM TRIS PH8.0, 0.4MM EDTA AND 100MM TRIS PH8.0, 200MM NACL, 10MM MG(OAC)2, pH 8.00
結晶化
*PLUS
温度: 14 ℃ / 手法: 蒸気拡散法, ハンギングドロップ法
溶液の組成
*PLUS
ID
濃度
一般名
Crystal-ID
Sol-ID
詳細
化学式
1
6mg/ml
protein
1
drop
2
50mM
Tris
1
drop
pH8.0
3
300mM
1
drop
NaCl
4
5mM
1
drop
Mg(OAc)2
5
0.2mM
EDTA
1
drop
6
10 %(v/v)
glycerol
1
drop
7
100mM
Tris
1
reservoir
pH8.0
8
200mM
1
reservoir
NaCl
9
10mM
1
reservoir
Mg(OAc)2
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: SRS / ビームライン: PX9.6 / 波長: 0.87
検出器
タイプ: ADSC CCD / 検出器: CCD / 日付: 2001年3月3日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.87 Å / 相対比: 1
反射
解像度: 2.2→30 Å / Num. obs: 39106 / % possible obs: 93 % / 冗長度: 1.9 % / Biso Wilson estimate: 31.5 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 8
反射 シェル
解像度: 2.2→2.31 Å / 冗長度: 1.8 % / Rmerge(I) obs: 0.15 / Mean I/σ(I) obs: 4.2 / % possible all: 92.6
解像度: 2.2→28.95 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1072988.9 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 立体化学のターゲット値: MAXIMUM LIKELIHOOD USING AMPLITUDES 詳細: NO DENSITY WAS FOUND FOR THE LOOPS A249 - A262 B249 - B261 C249 - C262 D249 - D268 NO DENSITY WAS FOUND FOR SEVERAL SIDECHAINS. THESE WERE TRUNCATED AT CB.