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Yorodumi- PDB-1gjf: Peptide Antagonist of IGFBP1, (i,i+7) Covalently Restrained Analo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gjf | ||||||
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Title | Peptide Antagonist of IGFBP1, (i,i+7) Covalently Restrained Analog, Minimized Average Structure | ||||||
Components | IGFBP-1 antagonist | ||||||
Keywords | ANTAGONIST / covalently constrained helix | ||||||
Function / homology | PENTANE Function and homology information | ||||||
Method | SOLUTION NMR / restrained molecular dynamics | ||||||
Authors | Skelton, N.J. / Chen, Y.M. / Dubree, N. / Quan, C. / Jackson, D.Y. / Cochran, A.G. / Zobel, K. / Deshayes, K. / Baca, M. / Pisabarro, M.T. / Lowman, H.B. | ||||||
Citation | Journal: Biochemistry / Year: 2001 Title: Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1. Authors: Skelton, N.J. / Chen, Y.M. / Dubree, N. / Quan, C. / Jackson, D.Y. / Cochran, A. / Zobel, K. / Deshayes, K. / Baca, M. / Pisabarro, M.T. / Lowman, H.B. #1: Journal: Biochemistry / Year: 1998 Title: Molecular Mimics of Insulin-like Growth Factor 1 (IGF-1) for Inhibiting IGF-1: IGF-Binding Protein Interactions Authors: Lowman, H.B. / Chen, Y.M. / Skelton, N.J. / Mortensen, D.L. / Tomlinson, E.E. / Sadick, M.D. / Robinson, I.C. / Clark, R.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gjf.cif.gz | 13.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gjf.ent.gz | 7.3 KB | Display | PDB format |
PDBx/mmJSON format | 1gjf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1gjf_validation.pdf.gz | 297.9 KB | Display | wwPDB validaton report |
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Full document | 1gjf_full_validation.pdf.gz | 297.7 KB | Display | |
Data in XML | 1gjf_validation.xml.gz | 1.8 KB | Display | |
Data in CIF | 1gjf_validation.cif.gz | 1.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gj/1gjf ftp://data.pdbj.org/pub/pdb/validation_reports/gj/1gjf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1642.879 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. It was designed from sequence selected from a phage display library. |
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#2: Chemical | ChemComp-LNK / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: restrained molecular dynamics / Software ordinal: 1 Details: The structure was detemined on the basis of 138 NOE distance restraints and 11 dihedral angle restraints. The resulting ensemble had no restraint violations greater than 0.07 Angstroms or 1. ...Details: The structure was detemined on the basis of 138 NOE distance restraints and 11 dihedral angle restraints. The resulting ensemble had no restraint violations greater than 0.07 Angstroms or 1.4 deg. The mean restraint violation energy was 0.04 +/- 0.03 kcal/mol. | ||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |