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- PDB-1gis: A TRICHOSANTHIN(TCS) MUTANT(E85Q) COMPLEX STRUCTURE WITH 2'-DEOXY... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gis | |||||||||
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Title | A TRICHOSANTHIN(TCS) MUTANT(E85Q) COMPLEX STRUCTURE WITH 2'-DEOXY-ADENOSIN-5'-MONOPHOSPHATE | |||||||||
![]() | RIBOSOME-INACTIVATING PROTEIN ALPHA-TRICHOSANTHIN | |||||||||
![]() | HYDROLASE / PROTEIN-SUB COMPLEX / TRICHOSANTHIN / TCS | |||||||||
Function / homology | ![]() regulation of defense response to virus / rRNA N-glycosylase / rRNA N-glycosylase activity / defense response / toxin activity / negative regulation of translation Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Guo, Q. / Liu, Y. / Dong, Y. / Rao, Z. | |||||||||
![]() | ![]() Title: Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants. Authors: Guo, Q. / Zhou, W. / Too, H.M. / Li, J. / Liu, Y. / Bartlam, M. / Dong, Y. / Wong, K.B. / Shaw, P.C. / Rao, Z. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.6 KB | Display | ![]() |
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PDB format | ![]() | 49.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 27296.980 Da / Num. of mol.: 1 / Mutation: E86Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-D5M / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.59 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: evaporation / pH: 5.7 Details: CaCl2, KCl, NaAC-HAC, pH 5.7, EVAPORATION, temperature 291.0K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Dec 28, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. all: 25659 / Num. obs: 24510 / % possible obs: 95.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Biso Wilson estimate: 11.5 Å2 / Rmerge(I) obs: 0.061 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 1.7→1.76 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.236 / Num. unique all: 2392 / % possible all: 95.6 |
Reflection | *PLUS Lowest resolution: 50 Å |
Reflection shell | *PLUS % possible obs: 95.6 % |
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Processing
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Refinement | Resolution: 1.7→50 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: CNS
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Refinement step | Cycle: LAST / Resolution: 1.7→50 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 30 Å | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |