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TitleSubstrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants.
Journal, issue, pagesProtein Eng., Vol. 16, Page 391-396, Year 2003
Publish dateMar 15, 2001 (structure data deposition date)
AuthorsGuo, Q. / Zhou, W. / Too, H.M. / Li, J. / Liu, Y. / Bartlam, M. / Dong, Y. / Wong, K.B. / Shaw, P.C. / Rao, Z.
External linksProtein Eng. / PubMed:12874371
MethodsX-ray diffraction
Resolution1.7 - 1.8 Å
Structure data

PDB-1gis:
A TRICHOSANTHIN(TCS) MUTANT(E85Q) COMPLEX STRUCTURE WITH 2'-DEOXY-ADENOSIN-5'-MONOPHOSPHATE
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-1giu:
A TRICHOSANTHIN(TCS) MUTANT(E85R) COMPLEX STRUCTURE WITH ADENINE
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-D5M:
2'-DEOXYADENOSINE-5'-MONOPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-ADE:
ADENINE

Source
  • trichosanthes kirilowii (Chinese cucumber)
KeywordsHYDROLASE / PROTEIN-SUB COMPLEX / TRICHOSANTHIN / TCS

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