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- PDB-1g8y: CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF P... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1g8y | ||||||
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Title | CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010 | ||||||
![]() | REGULATORY PROTEIN REPA | ||||||
![]() | TRANSCRIPTION / P-loop | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Niedenzu, T. / Roeleke, D. / Bains, G. / Scherzinger, E. / Saenger, W. | ||||||
![]() | ![]() Title: Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010. Authors: Niedenzu, T. / Roleke, D. / Bains, G. / Scherzinger, E. / Saenger, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 547.8 KB | Display | ![]() |
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PDB format | ![]() | 453.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.4 KB | Display | ![]() |
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Full document | ![]() | 511.7 KB | Display | |
Data in XML | ![]() | 60.7 KB | Display | |
Data in CIF | ![]() | 93.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is one of the two hexamers in the asymmetric unit: chains a-f or g-l |
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Components
#1: Protein | Mass: 29945.189 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 5000 monomethyl ether, 2-methyl-2,4-pentanediol, NaCl, sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 22, 1998 |
Radiation | Monochromator: bent single-crystal germanium triangular monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9059 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. all: 149192 / Num. obs: 149192 / % possible obs: 94.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 2.53 % / Biso Wilson estimate: 26.8 Å2 / Rmerge(I) obs: 0.037 / Net I/σ(I): 18.9 |
Reflection shell | Resolution: 2.4→2.46 Å / Redundancy: 2.46 % / Rmerge(I) obs: 0.117 / Mean I/σ(I) obs: 6.9 / Num. unique all: 8579 / % possible all: 81.7 |
Reflection | *PLUS Num. measured all: 377676 / Rmerge(I) obs: 0.034 |
Reflection shell | *PLUS % possible obs: 81.7 % |
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Processing
Software |
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Refinement | Method to determine structure: ![]()
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Displacement parameters |
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.4→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.49 Å / Rfactor Rfree error: 0.02
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 30 Å / σ(F): 0 / % reflection Rfree: 2.1 % / Rfactor obs: 0.221 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.5 | |||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor Rfree: 0.32 / Rfactor Rwork: 0.261 |