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Open data
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Basic information
Entry | Database: PDB / ID: 1g70 | ||||||
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Title | COMPLEX OF HIV-1 RRE-IIB RNA WITH RSG-1.2 PEPTIDE | ||||||
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![]() | VIRAL PROTEIN/RNA / peptide-RNA complex / non-canonical base pairs / VIRAL PROTEIN-RNA COMPLEX | ||||||
Function / homology | RNA / RNA (> 10) / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
![]() | Gosser, Y. / Hermann, T. / Majumdar, A. / Hu, W. / Frederick, R. / Jiang, F. / Xu, W. / Patel, D.J. | ||||||
![]() | ![]() Title: Peptide-triggered conformational switch in HIV-1 RRE RNA complexes. Authors: Gosser, Y. / Hermann, T. / Majumdar, A. / Hu, W. / Frederick, R. / Jiang, F. / Xu, W. / Patel, D.J. #1: ![]() Title: Molding a peptide into an RNA site by in vivo peptide evolution Authors: Harada, K. / Martin, S.S. / Tan, R. / Frankel, A.D. #2: ![]() Title: alpha Helix-RNA major groove recognition in an HIV-1 Rev peptide-RRE RNA complex Authors: Battiste, J.L. / Mao, H. / Rao, N.S. / Tan, R. / Muhandiram, D.R. / Kay, L.E. / Frankel, A.D. / Williamson, J.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 356.3 KB | Display | ![]() |
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PDB format | ![]() | 294.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 10343.173 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Protein/peptide | Mass: 2574.918 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D homonuclear experiments, 2D, 3D heteronuclear experiments |
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Sample preparation
Sample conditions | Ionic strength: 10mM Sodium phosphate, 12.5mM Sodium acetate, 25mM Sodium chloride, 0.1mM EDTA pH: 6 / Pressure: ambient / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry,structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 90 / Conformers submitted total number: 14 |